RRC ID |
37882
|
著者 |
Matsuura K, Seta H, Haraguchi Y, Alsayegh K, Sekine H, Shimizu T, Hagiwara N, Yamazaki K, Okano T.
|
タイトル |
TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues.
|
ジャーナル |
Sci Rep
|
Abstract |
The development of a suitable strategy for eliminating remaining undifferentiated cells is indispensable for the use of human-induced pluripotent stem (iPS) cell-derived cells in regenerative medicine. Here, we show for the first time that TRPV-1 activation through transient culture at 42 °C in combination with agonists is a simple and useful strategy to eliminate iPS cells from bioengineered cardiac cell sheet tissues. When human iPS cells were cultured at 42 °C, almost all cells disappeared by 48 hours through apoptosis. However, iPS cell-derived cardiomyocytes and fibroblasts maintained transcriptional and protein expression levels, and cardiac cell sheets were fabricated after reducing the temperature. TRPV-1 expression in iPS cells was upregulated at 42 °C, and iPS cell death at 42 °C was TRPV-1-dependent. Furthermore, TRPV-1 activation through thermal or agonist treatment eliminated iPS cells in cardiac tissues for a final concentration of 0.4% iPS cell contamination. These findings suggest that the difference in tolerance to TRPV-1 activation between iPS cells and iPS cell-derived cardiac cells could be exploited to eliminate remaining iPS cells in bioengineered cell sheet tissues, which will further reduce the risk of tumour formation.
|
巻・号 |
6
|
ページ |
21747
|
公開日 |
2016-2-18
|
DOI |
10.1038/srep21747
|
PII |
srep21747
|
PMID |
26888607
|
PMC |
PMC4757885
|
MeSH |
Cell Differentiation
Cells, Cultured
Coculture Techniques
Fibroblasts / cytology
Fibroblasts / metabolism
Hot Temperature
Humans
Induced Pluripotent Stem Cells / cytology*
Myocytes, Cardiac / cytology*
Myocytes, Cardiac / metabolism
TRPV Cation Channels / agonists
TRPV Cation Channels / genetics*
TRPV Cation Channels / metabolism*
Tissue Engineering / methods*
|
IF |
3.998
|
引用数 |
14
|
WOS 分野
|
CELL BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
201B7(HPS0063)
253G1(HPS0002) |