RRC ID 46838
著者 Nguyen MB, Vuong LT, Choi KW.
タイトル Ebi modulates wing growth by ubiquitin-dependent downregulation of Crumbs in Drosophila.
ジャーナル Development
Abstract Notch signaling at the dorsoventral (DV) boundary is essential for patterning and growth of wings in Drosophila The WD40 domain protein Ebi has been implicated in the regulation of Notch signaling at the DV boundary. Here we show that Ebi regulates wing growth by antagonizing the function of the transmembrane protein Crumbs (Crb). Ebi physically binds to the extracellular domain of Crb (Crbext), and this interaction is specifically mediated by WD40 repeats 7-8 of Ebi and a laminin G domain of Crbext Wing notching resulting from reduced levels of Ebi is suppressed by decreasing the Crb function. Consistent with this antagonistic genetic relationship, Ebi knockdown in the DV boundary elevates the Crb protein level. Furthermore, we show that Ebi is required for downregulation of Crb by ubiquitylation. Taken together, we propose that the interplay of Crb expression in the DV boundary and ubiquitin-dependent Crb downregulation by Ebi provides a mechanism for the maintenance of Notch signaling during wing development.
巻・号 143(19)
ページ 3506-3513
公開日 2016-10-1
DOI 10.1242/dev.142059
PII 143/19/3506
PMID 27702784
MeSH Animals Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Down-Regulation / genetics Down-Regulation / physiology Drosophila Drosophila Proteins / genetics Drosophila Proteins / metabolism* GTP-Binding Proteins / genetics GTP-Binding Proteins / metabolism* Gene Expression Regulation, Developmental / genetics Gene Expression Regulation, Developmental / physiology Membrane Proteins / genetics Membrane Proteins / metabolism* Receptors, Notch / genetics Receptors, Notch / metabolism Signal Transduction / genetics Signal Transduction / physiology Ubiquitin / genetics Ubiquitin / metabolism* WD40 Repeats / genetics WD40 Repeats / physiology Wings, Animal / embryology* Wings, Animal / metabolism*
IF 5.611
引用数 3
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ 4063R-2