RRC ID 40969
著者 Jung WY, Lee SH, Jin HM, Jeon CO.
タイトル Lentibacillus garicola sp. nov., isolated from myeolchi-aekjeot, a Korean fermented anchovy sauce.
ジャーナル Antonie Van Leeuwenhoek
Abstract A Gram-stain positive, aerobic and moderately halophilic bacterium, designated strain SL-MJ1(T), was isolated from myeolchi-aekjeot, a Korean traditionally fermented anchovy sauce. Cells were observed to be non-spore-forming rods showing oxidase-negative and catalase-positive reactions. Growth of strain SL-MJ1(T) was observed at 15-40 °C (optimum, 30 °C) and pH 6.5-8.0 (optimum, pH 7.0) and in the presence of 0-20 % (w/v) NaCl (optimum, 6 %). Chemotaxonomic data (major isoprenoid quinone: menaquinone-7; DNA G + C content: 42.8 mol%; cell wall type: meso-diaminopimelic acid; major fatty acids: anteiso-C15:0, anteiso-C17:0 and iso-C16:0) support the affiliation of the isolate to the genus Lentibacillus. Phosphatidylglycerol, diphosphatidylglycerol and an unidentified phospholipid were identified as the major cellular polar lipids. Comparative 16S rRNA gene sequence analysis showed that strain SL-MJ1(T) is most closely related to Lentibacillus juripiscarius IS40-3(T) with a 95.3 % similarity. Phylogenetic analysis based on 16S rRNA gene sequences also support the conclusion that strain SL-MJ1(T) forms a phylogenetic lineage with members of the genus Lentibacillus within the family Bacillaceae. Given its phylogenetic, phenotypic and chemotaxonomic features, we conclude that strain SL-MJ1(T) represents a novel species of the genus Lentibacillus, for which the name Lentibacillus garicola sp. nov. is proposed. The type strain is SL-MJ1(T) (=KACC 18130(T) = JCM 30131(T)).
巻・号 107(6)
ページ 1569-76
公開日 2015-6-1
DOI 10.1007/s10482-015-0450-2
PMID 25851835
MeSH Aerobiosis Bacillaceae / classification* Bacillaceae / genetics Bacillaceae / isolation & purification* Bacillaceae / physiology Bacterial Typing Techniques Base Composition Cell Wall / chemistry Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Diaminopimelic Acid / analysis Fatty Acids / analysis Food Microbiology* Hydrogen-Ion Concentration Korea Molecular Sequence Data Phospholipids / analysis Phylogeny Quinones / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride / metabolism Temperature
IF 2.674
引用数 7
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 30131 JCM 12147