| RRC ID |
41023
|
| 著者 |
Fang B, Liu C, Guan X, Song J, Zhao J, Liu H, Li C, Ning W, Wang X, Xiang W.
|
| タイトル |
Two new species of the genus Micromonospora: Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. isolated from the insects.
|
| ジャーナル |
Antonie Van Leeuwenhoek
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| Abstract |
Two novel actinobacteria, strains NEAU-CX1(T) and NEAU-JC6(T), were isolated from nymphs of stinkbug (Palomena viridissima Poda) and a beetle (Harpalus sinicus Hope), respectively, collected from Harbin, Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The phylogenetic analysis based on 16S rRNA gene sequence of strain NEAU-CX1(T) showed it to be most closely related to Micromonospora coxensis JCM 13248(T) (99.3 % sequence similarity), Micromonospora purpureochromogenes DSM 43821(T) (99.1 %) and Micromonospora halophytica JCM 3125(T) (98.6 %), and that of strain NEAU-JC6(T) to Micromonospora haikouensis DSM 45626(T) (99.3 %), Micromonospora carbonacea JCM 3139(T) (99.1 %) and Micromonospora krabiensis JCM 12869(T) (99.1 %). The phylogenetic analysis based on gyrB gene sequence of strain NEAU-CX1(T) showed it to be most closely related to M. purpureochromogenes DSM 43821(T) (98.0 % sequence similarity), and that of strain NEAU-JC6(T) to M. haikouensis DSM 45626(T) (99.0 %) and M. carbonacea JCM 3139(T) (98.0 %). A combination of DNA-DNA hybridization results and cultural and physiological properties indicated that the two strains can be distinguished from their closest phylogenetic relatives. Thus, strains NEAU-CX1(T) and NEAU-JC6(T) represent two novel species of the genus Micromonospora, for which the names Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. are proposed. The type strains are NEAU-CX1(T) (=CGMCC 4.7175(T) = JCM 30056(T)) and NEAU-JC6(T) (=CGMCC 4.7173(T) = JCM 30055(T)).
|
| 巻・号 |
108(1)
|
| ページ |
141-50
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| 公開日 |
2015-7-1
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| DOI |
10.1007/s10482-015-0472-9
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| PMID |
25957972
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| MeSH |
Animals
Bacterial Typing Techniques
China
Cluster Analysis
DNA Gyrase / genetics
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Fatty Acids / analysis
Insecta / microbiology*
Micromonospora / classification*
Micromonospora / genetics
Micromonospora / isolation & purification*
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| IF |
2.674
|
| 引用数 |
14
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 13248
JCM 3139
JCM 12869
JCM 3125
JCM 30055
JCM 30056 |
