RRC ID 41024
著者 Wang X, Liu C, Cheng J, Zhang Y, Ma Z, Li L, Wang X, Xiang W.
タイトル Sphaerisporangium corydalis sp. nov., isolated from the root of Corydalis yanhusuo L.
ジャーナル Antonie Van Leeuwenhoek
Abstract Two Gram-stain positive, aerobic actinomycete strains, designated NEAU-YHS12 and NEAU-YHS15(T), were isolated from the root of Corydalis yanhusuo L. collected from Wuchang, Heilongjiang Province, northeast China. Phylogenetic analysis of 16S rRNA gene sequences showed that the two strains are closely related to one another (99.8 % similarity), and had the closest relationship with Sphaerisporangium cinnabarinum JCM 3291(T) (98.7, 98.6 %), Sphaerisporangium flaviroseum YIM 48771(T) (98.6, 98.6 %), Sphaerisporangium melleum JCM 13064(T) (98.5, 98.4 %) and Sphaerisporangium dianthi NEAU-CY18(T) (98.4, 98.4 %). DNA-DNA hybridization value between strains NEAU-YHS12 and NEAU-YHS15(T) was 82 ± 1.4 %, and the values between the two strains and the closely related type strains were well below 70 %. The two strains also shared a number of phenotypic characteristics that were distinct from the closely related species. Both strains were observed to contain MK-9(H6), MK-9(H4) and MK-9(H2) as the detected menaquinones. The cell wall peptidoglycan was found to contain meso-diaminopimelic acid. The phospholipid profiles were found to contain diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, phosphoglycolipid, and an unidentified phospholipid. The major fatty acids were identified as iso-C16:0, C17:1 ω7c, C18:0 and iso-C15:0. On the basis of the genetic and phenotypic properties, it is proposed that strains NEAU-YHS15(T) and NEAU-YHS12 be classified as representatives of a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium corydalis sp. nov is proposed. The type strain is NEAU-YHS15(T) (CGMCC 4.7148(T) = DSM46732(T)).
巻・号 108(1)
ページ 133-9
公開日 2015-7-1
DOI 10.1007/s10482-015-0471-x
PMID 25963939
MeSH Actinobacteria / classification* Actinobacteria / genetics Actinobacteria / isolation & purification* Actinobacteria / physiology Aerobiosis Cell Wall / chemistry China Cluster Analysis Corydalis / microbiology* Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Diaminopimelic Acid / analysis Fatty Acids / analysis Molecular Sequence Data Nucleic Acid Hybridization Peptidoglycan / analysis Phospholipids / analysis Phylogeny Plant Roots / microbiology RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Vitamin K 2 / analysis
IF 2.674
引用数 3
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 3291 JCM 13064