RRC ID 41090
著者 Chung EJ, Park TS, Kim KH, Jeon CO, Lee HI, Chang WS, Aslam Z, Chung YR.
タイトル Nitrospirillum irinus sp. nov., a diazotrophic bacterium isolated from the rhizosphere soil of Iris and emended description of the genus Nitrospirillum.
ジャーナル Antonie Van Leeuwenhoek
Abstract A polyphasic approach was used to characterize a novel nitrogen-fixing bacterial strain, designated YC6995(T), isolated from the rhizosphere soil of Iris ensata var. spontanea (Makino) Nakai inhabiting a wetland located at an altitude of 960 m on Jiri Mountain, Korea. Strain YC6995(T) cells were Gram-negative, and rod-shaped, with motility provided by a single polar flagellum. Optimal growth conditions were 30 °C and pH 7.0. The major fatty acids of strain YC6995(T) were C18:1 ω7c, C18:1 2-OH and C16:0 3-OH. The major respiratory quinone was ubiquinone-10 (Q-10). The polar lipids were phosphatidylethanolamine, phosphatidyldimethylethanolamine, phosphatidylcholine, phosphatidylglycerol and unidentified glycolipids. The genomic DNA G+C content was 64.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed strain YC6995(T) to form a phyletic lineage with Nitrospirillum amazonense DSM 2787(T) with a high sequence similarity (97.2 %), but it displayed low sequence similarity with other remotely related genera, including Azospirillum (<93 %), Rhodocista (93.1-93.4 %), and Skermanella (91.2-93.3 %) in the family Alphaproteobacteria. Based on the phenotypic, chemotaxonomic, and phylogenetic evidences, strain YC6995(T) represents a novel species within the genus Nitrospirillum, for which the name Nitrospirillum irinus sp. nov. is proposed. The type strain is YC6995(T) (= KACC 13777(T) = DSM 22198(T)). An emended description of the genus Nitrospirillum is also proposed.
巻・号 108(3)
ページ 721-9
公開日 2015-9-1
DOI 10.1007/s10482-015-0528-x
PMID 26155773
MeSH Bacterial Typing Techniques Base Composition Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Flagella / physiology Glycolipids / analysis Hydrogen-Ion Concentration Iris / microbiology Locomotion Molecular Sequence Data Nitrogen Fixation Phospholipids / analysis Phylogeny Quinones / analysis RNA, Ribosomal, 16S / genetics Republic of Korea Rhizosphere Rhodospirillaceae / classification* Rhodospirillaceae / genetics Rhodospirillaceae / isolation & purification* Rhodospirillaceae / physiology Sequence Analysis, DNA Soil Microbiology* Temperature
IF 2.674
引用数 3
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 21060