RRC ID 47456
著者 Funabashi T, Katoh Y, Michisaka S, Terada M, Sugawa M, Nakayama K.
タイトル Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal.
ジャーナル Mol Biol Cell
Abstract Cilia function as cellular antennae to sense and transduce extracellular signals. A number of proteins are specifically localized in cilia. Anterograde and retrograde ciliary protein trafficking are mediated by the IFT-B and IFT-A complexes in concert with kinesin-2 and dynein-2 motors, respectively. However, the role of KIF17, a homodimeric kinesin-2 protein, in protein trafficking has not been fully understood in vertebrate cilia. In this study, we demonstrated, by using the visible immunoprecipitation assay, that KIF17 interacts with the IFT46-IFT56 dimer in the IFT-B complex through its C-terminal sequence located immediately upstream of the nuclear localization signal (NLS). We then showed that KIF17 reaches the ciliary tip independently of its motor domain and requires IFT-B binding for its entry into cilia rather than for its intraciliary trafficking. We further showed that KIF17 ciliary entry depends not only on its binding to IFT-B but also on its NLS, to which importin α proteins bind. Taking the results together, we conclude that in mammalian cells, KIF17 is dispensable for ciliogenesis and IFT-B trafficking but requires IFT-B, as well as its NLS, for its ciliary entry across the permeability barrier located at the ciliary base.
巻・号 28(5)
ページ 624-633
公開日 2017-3-1
DOI 10.1091/mbc.E16-09-0648
PII mbc.E16-09-0648
PMID 28077622
PMC PMC5328621
MeSH Axoneme / metabolism Carrier Proteins / metabolism* Cell Line Cilia / metabolism* Dyneins / metabolism Flagella / metabolism Gene Knockout Techniques Humans Kinesins / metabolism* Molecular Motor Proteins / metabolism* Nuclear Localization Signals / metabolism Protein Transport / physiology Retinal Pigment Epithelium / cytology Retinal Pigment Epithelium / metabolism
IF 3.791
引用数 23
WOS 分野 CELL BIOLOGY
リソース情報
遺伝子材料 Genome Network Project human cDNA clone IRAK140P09 (HGX056369) pCAG-EGFP-C-KIF17 (RDB15196) pCAG-mCherry-C-KIF17 (RDB15197).