RRC ID 40086
著者 Takahashi-Nakaguchi A, Muraosa Y, Hagiwara D, Sakai K, Toyotome T, Watanabe A, Kawamoto S, Kamei K, Gonoi T, Takahashi H.
タイトル Genome sequence comparison of Aspergillus fumigatus strains isolated from patients with pulmonary aspergilloma and chronic necrotizing pulmonary aspergillosis.
ジャーナル Med Mycol
Abstract Aspergillus fumigatus is the Aspergillus species most commonly associated with aspergillosis. Of the various presentations of aspergillosis, one of the most frequently observed in cases involving A. fumigatus pulmonary infections is aspergilloma (PA). In such infections one finds a fungus ball composed of fungal hyphae, inflammatory cells, fibrin, mucus, and tissue debris. Chronic necrotizing pulmonary aspergillosis (CNPA), also known as semi-invasive or invasive aspergillosis, is locally invasive and predominantly seen in patients with mild immunodeficiency or with a chronic lung disease. In the present study, with the aid of a next-generation sequencer, we conducted whole genome sequence (WGS) analyses of 17 strains isolated from patients in Japan with PA and CNPA. A total of 99,088 SNPs were identified by mapping the reads to A. fumigatus genome reference strain Af293, and according to genome-wide phylogenetic analysis, there were no correlations between the whole genome sequence typing results and pathologic conditions of patients. Here, we conducted the first multi-genome WGS study to focus on the A. fumigatus strains isolated from patients with PA and CNPA, and comprehensively characterized genetic variations of strains. WGS approach will help in better understanding of molecular mechanisms of aspergillosis cases caused by A. fumigatus.
巻・号 53(4)
ページ 353-60
公開日 2015-5-1
DOI 10.1093/mmy/myv003
PII myv003
PMID 25851262
MeSH Aspergillus fumigatus / classification Aspergillus fumigatus / genetics* Aspergillus fumigatus / isolation & purification* DNA, Fungal / chemistry DNA, Fungal / genetics Genome, Fungal* High-Throughput Nucleotide Sequencing Humans Japan Pulmonary Aspergillosis / microbiology* Pulmonary Aspergillosis / pathology*
IF 2.822
引用数 12
WOS 分野 VETERINARY SCIENCES MYCOLOGY INFECTIOUS DISEASES
リソース情報
病原微生物 IFM 55369 IFM 58026 IFM 58029 IFM 58401 IFM 59056 IFM 59073 IFM 59359 IFM 59361 IFM 59365 IFM 59777 IFM 60514 IFM 61118 IFM 61407 IFM 61578 IFM 61610 IFM 62115 IFM 62516