RRC ID 46776
著者 Toyotome T, Takahashi H, Kamei K.
タイトル MEIS3 is repressed in A549 lung epithelial cells by deoxynivalenol and the repression contributes to the deleterious effect.
ジャーナル J Toxicol Sci
Abstract Deoxynivalenol (DON) is an important Fusarium toxin of concern for food safety. The inhalation of powder contaminated with DON is possible and may cause lung toxicity. In this study, we analyzed the gene expression profile of A549 cells treated for 24 hr with 0.2 µg/mL DON by microarray analysis. In total, 16 genes and 5 noncoding RNAs were significantly affected by DON treatment. The repression of B3GALT4, MEIS3, AK7, SEMA3A, KCNMB4, and SCARA5 was confirmed by quantitative PCR. We investigated the DON toxicity on A549 cells that exogenously expressed these 6 genes. The result indicated that A549 cells that transiently expressed MEIS3 were tolerant to the deleterious effects of DON. Our data show that DON affected the expression of genes with various functions, and suggest that the repression of MEIS3 plays roles in the deleterious effect of DON in A549 lung epithelial cells.
巻・号 41(1)
ページ 25-31
公開日 2016-2-1
DOI 10.2131/jts.41.25
PMID 26763390
MeSH Cell Line Epigenetic Repression / drug effects* Epigenetic Repression / genetics Epithelial Cells / metabolism* Homeodomain Proteins / genetics* Homeodomain Proteins / metabolism* Humans Lung / cytology Polymerase Chain Reaction Tissue Array Analysis Transcription Factors / genetics* Transcription Factors / metabolism* Transcriptome / drug effects* Trichothecenes / pharmacology*
IF 1.737
引用数 0
WOS 分野 TOXICOLOGY
リソース情報
病原微生物