RRC ID 25666
著者 Kawahara-Miki R, Sano S, Nunome M, Shimmura T, Kuwayama T, Takahashi S, Kawashima T, Matsuda Y, Yoshimura T, Kono T.
タイトル Next-generation sequencing reveals genomic features in the Japanese quail.
ジャーナル Genomics
Abstract The Japanese quail has several advantages as a laboratory animal for biological and biomedical investigations. In this study, the draft genome of the Japanese quail was sequenced and assembled using next-generation sequencing technology. To improve the quality of the assembly, the sequence reads from the Japanese quail were aligned against the reference genome of the chicken. The final draft assembly consisted of 1.75 Gbp with an N50 contig length of 11,409 bp. On the basis of the draft genome sequence obtained, we developed 100 microsatellite markers and used these markers to evaluate the genetic variability and diversity of 11 lines of Japanese quail. Furthermore, we identified Japanese quail orthologs of spermatogenesis markers and analyzed their expression using in situ hybridization. The Japanese quail genome sequence obtained in the present study could enhance the value of this species as a model animal.
巻・号 101(6)
ページ 345-53
公開日 2013-6-1
DOI 10.1016/j.ygeno.2013.03.006
PII S0888-7543(13)00059-1
PMID 23557672
MeSH Animals Base Sequence Coturnix / genetics* Genome* High-Throughput Nucleotide Sequencing* Molecular Sequence Annotation Molecular Sequence Data Open Reading Frames Sequence Analysis, DNA*
IF 6.205
引用数 44
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
リソース情報
ニワトリ・ウズラ