RRC ID 47696
著者 Wu J, Platero-Luengo A, Sakurai M, Sugawara A, Gil MA, Yamauchi T, Suzuki K, Bogliotti YS, Cuello C, Morales Valencia M, Okumura D, Luo J, Vilariño M, Parrilla I, Soto DA, Martinez CA, Hishida T, Sánchez-Bautista S, Martinez-Martinez ML, Wang H, Nohalez A, Aizawa E, Martinez-Redondo P, Ocampo A, Reddy P, Roca J, Maga EA, Esteban CR, Berggren WT, Nuñez Delicado E, Lajara J, Guillen I, Guillen P, Campistol JM, Martinez EA, Ross PJ, Izpisua Belmonte JC.
タイトル Interspecies Chimerism with Mammalian Pluripotent Stem Cells.
ジャーナル Cell
Abstract Interspecies blastocyst complementation enables organ-specific enrichment of xenogenic pluripotent stem cell (PSC) derivatives. Here, we establish a versatile blastocyst complementation platform based on CRISPR-Cas9-mediated zygote genome editing and show enrichment of rat PSC-derivatives in several tissues of gene-edited organogenesis-disabled mice. Besides gaining insights into species evolution, embryogenesis, and human disease, interspecies blastocyst complementation might allow human organ generation in animals whose organ size, anatomy, and physiology are closer to humans. To date, however, whether human PSCs (hPSCs) can contribute to chimera formation in non-rodent species remains unknown. We systematically evaluate the chimeric competency of several types of hPSCs using a more diversified clade of mammals, the ungulates. We find that naïve hPSCs robustly engraft in both pig and cattle pre-implantation blastocysts but show limited contribution to post-implantation pig embryos. Instead, an intermediate hPSC type exhibits higher degree of chimerism and is able to generate differentiated progenies in post-implantation pig embryos.
巻・号 168(3)
ページ 473-486.e15
公開日 2017-1-26
DOI 10.1016/j.cell.2016.12.036
PII S0092-8674(16)31752-4
PMID 28129541
PMC PMC5679265
MeSH Animals Blastocyst CRISPR-Cas Systems Cattle Chimerism* Embryo, Mammalian / cytology Female Gene Editing* Humans Male Mammals / classification Mammals / embryology* Mice Mice, Inbred C57BL Mice, Inbred ICR Pluripotent Stem Cells Rats Rats, Sprague-Dawley Sus scrofa
IF 38.637
引用数 186
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 iPS-MEF-Ng-20D-17(APS0001)