RRC ID 47269
著者 Toselli-Mollereau E, Robellet X, Fauque L, Lemaire S, Schiklenk C, Klein C, Hocquet C, Legros P, N'Guyen L, Mouillard L, Chautard E, Auboeuf D, Haering CH, Bernard P.
タイトル Nucleosome eviction in mitosis assists condensin loading and chromosome condensation.
ジャーナル EMBO J
Abstract Condensins associate with DNA and shape mitotic chromosomes. Condensins are enriched nearby highly expressed genes during mitosis, but how this binding is achieved and what features associated with transcription attract condensins remain unclear. Here, we report that condensin accumulates at or in the immediate vicinity of nucleosome-depleted regions during fission yeast mitosis. Two transcriptional coactivators, the Gcn5 histone acetyltransferase and the RSC chromatin-remodelling complex, bind to promoters adjoining condensin-binding sites and locally evict nucleosomes to facilitate condensin binding and allow efficient mitotic chromosome condensation. The function of Gcn5 is closely linked to condensin positioning, since neither the localization of topoisomerase II nor that of the cohesin loader Mis4 is altered in gcn5 mutant cells. We propose that nucleosomes act as a barrier for the initial binding of condensin and that nucleosome-depleted regions formed at highly expressed genes by transcriptional coactivators constitute access points into chromosomes where condensin binds free genomic DNA.
巻・号 35(14)
ページ 1565-81
公開日 2016-7-15
DOI 10.15252/embj.201592849
PII embj.201592849
PMID 27266525
PMC PMC4946138
MeSH Acetyltransferases / metabolism Adenosine Triphosphatases / metabolism* Base Composition Chromosomes, Fungal / metabolism* DNA-Binding Proteins / metabolism* Mitosis* Multiprotein Complexes / metabolism* Nucleosomes / metabolism* Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / metabolism Transcription Factors / metabolism
IF 9.889
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
酵母 cnd2-GFP-LEU2?