RRC ID 41159
著者 Hoang VA, Kim YJ, Nguyen NL, Kim SK, Yang DC.
タイトル Lactobacillus vespulae sp. nov., isolated from gut of a queen wasp (Vespula vulgaris).
ジャーナル Int J Syst Evol Microbiol
Abstract A Gram-stain-positive, oxidase- and catalase-negative, rod-shaped, facultatively anaerobic bacterial strain, DCY75T, was isolated from a queen wasp (Vespula vulgaris). Growth occurred at 4–37 °C (optimum, 30 °C), at pH 3.5–8.0 (optimum, pH 5.0–6.0) and with ≤ 7.0 % (w/v) NaCl. Strain DCY75T produced gas during growth on glucose. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DCY75T belonged to the genus Lactobacillus and was closely related to Lactobacillus sanfranciscensis ATCC 27651T and Lactobacillus lindneri DSM 20690T at sequence similarities of 96.7 and 96.4 %, respectively. A comparison of two housekeeping genes, pheS and rpoA, revealed that strain DCT75T was well separated from other species of the genus Lactobacillus. Strain DCY75T produced d- and l-lactic acid isomers in a ratio of 22.5 : 77.5 (v/v). The major fatty acids were summed feature 8 (comprising C18 :  1ω7c and/or C18 : 1ω6c), C16 : 0, C18 : 1ω9c and C18 : 0.The peptidoglycan structure was of the A4α (l-Lys–d-Asp) type. Cell-wall sugars were glucose, galactose and ribose. The DNA G+C content was 35.5 ± 1.3 mol%. Based on phenotypic and genotypic properties, strain DCY75T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus vespulae sp. nov. is proposed. The type strain is DCY75T ( = KCTC 21023T = JCM 19742T).
巻・号 65(10)
ページ 3326-3332
公開日 2015-10-1
DOI 10.1099/ijsem.0.000420
PMID 26297032
MeSH Animals Bacterial Typing Techniques Base Composition Cell Wall / chemistry DNA, Bacterial / genetics Fatty Acids / chemistry Female Gastrointestinal Tract / microbiology* Lactobacillus / classification* Lactobacillus / genetics Lactobacillus / isolation & purification Molecular Sequence Data Peptidoglycan / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Republic of Korea Sequence Analysis, DNA Wasps / microbiology*
IF 2.415
引用数 3
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 19742