RRC ID 41237
著者 Dekio I, Culak R, Misra R, Gaulton T, Fang M, Sakamoto M, Ohkuma M, Oshima K, Hattori M, Klenk HP, Rajendram D, Gharbia SE, Shah HN.
タイトル Dissecting the taxonomic heterogeneity within Propionibacterium acnes: proposal for Propionibacterium acnes subsp. acnes subsp. nov. and Propionibacterium acnes subsp. elongatum subsp. nov.
ジャーナル Int J Syst Evol Microbiol
Abstract Propionibacterium acnes subsp. acnes subsp. nov. and Propionibacterium acnes subsp. elongatum subsp. nov. are described. These emanate from the three known phylotypes of P. acnes, designated types I, II and III. Electron microscopy confirmed the filamentous cell shape of type III, showing a striking difference from types I/II, which were short rods. Biochemical tests indicated that, in types I/II, either the pyruvate, l-pyrrolidonyl arylamidase or d-ribose 2 test was positive, whereas all of these were negative among type III strains. Matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) spectra, which profile mainly their ribosomal proteins, were different between these two groups. Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) spectra of all phylotypes revealed a specific protein biomarker that was overexpressed in type III strains compared with types I/II only when grown aerobically. Reference strains had high whole-genome similarity between types I (>91 %) and II (>75 %), but a considerably lower level of 72 % similarity with type III. recA and gyrB sequence dendrograms confirmed the distant relatedness of type III, indicating the presence of two distinct centres of variation within the species P. acnes. On the other hand, cellular fatty acid profiles and 16S rRNA gene sequence relatedness (>99.3 %) circumscribed the species. Thus, we propose two subspecies, Propionibacterium acnes subsp. acnes subsp. nov. for types I/II and Propionibacterium acnes subsp. elongatum subsp. nov. for type III. The type strain of Propionibacterium acnes subsp. acnes is NCTC 737T ( = ATCC 6919T = JCM 6425T = DSM 1897T = CCUG 1794T), while the type strain of Propionibacterium acnes subsp. elongatum is K124T ( = NCTC 13655T = JCM 18919T).
巻・号 65(12)
ページ 4776-4787
公開日 2015-12-1
DOI 10.1099/ijsem.0.000648
PMID 26432704
MeSH Bacterial Proteins / chemistry Bacterial Typing Techniques DNA, Bacterial / genetics Fatty Acids / chemistry Genes, Bacterial Humans Molecular Sequence Data Nucleic Acid Hybridization Phylogeny* Propionibacterium acnes / classification* RNA, Ribosomal, 16S / genetics Ribosomal Proteins / chemistry Sequence Analysis, DNA Skin / microbiology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
IF 2.415
引用数 29
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 6495 JCM 6473 JCM 18907 JCM 18909 JCM 18916 JCM 18918 JCM 18919 JCM 18920 JCM 18925 JCM 18926 JCM 21245