RRC ID |
30587
|
著者 |
Iitsuka T, Mita K, Hozumi A, Hamada M, Satoh N, Sasakura Y.
|
タイトル |
Transposon-mediated targeted and specific knockdown of maternally expressed transcripts in the ascidian Ciona intestinalis.
|
ジャーナル |
Sci Rep
|
Abstract |
Maternal mRNAs play crucial roles during early embryogenesis of ascidians, but their functions are largely unknown. In this study, we developed a new method to specifically knockdown maternal mRNAs in Ciona intestinalis using transposon-mediated transgenesis. We found that GFP expression is epigenetically silenced in Ciona intestinalis oocytes and eggs, and this epigenetic silencing of GFP was used to develop the knockdown method. When the 5' upstream promoter and 5' untranslated region (UTR) of a maternal gene are used to drive GFP in eggs, the maternal gene is specifically knocked down together with GFP. The 5' UTR of the maternal gene is the major element that determines the target gene silencing. Zygotic transcription of the target gene is unaffected, suggesting that the observed phenotypes specifically reflect the maternal function of the gene. This new method can provide breakthroughs in studying the functions of maternal mRNAs.
|
巻・号 |
4
|
ページ |
5050
|
公開日 |
2014-5-23
|
DOI |
10.1038/srep05050
|
PII |
srep05050
|
PMID |
24854849
|
PMC |
PMC4031475
|
MeSH |
5' Untranslated Regions / genetics*
Animals
Animals, Genetically Modified / genetics*
Animals, Genetically Modified / growth & development
Ciona intestinalis / genetics*
Ciona intestinalis / growth & development
DNA Methylation
DNA Transposable Elements / genetics*
Egg Proteins / antagonists & inhibitors
Egg Proteins / genetics*
Embryo, Nonmammalian / cytology
Embryo, Nonmammalian / metabolism
Female
Gene Expression Regulation, Developmental
Gene Silencing*
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
In Situ Hybridization
RNA Stability
RNA, Messenger / antagonists & inhibitors
RNA, Messenger / genetics*
|
IF |
3.998
|
引用数 |
6
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
リソース情報 |
カタユウレイボヤ・(ニッポンウミシダ) |
Wild C. int
Tg[MiCiTnIGCimTG]1
Tg[MiCiTnIGCipemG]2
Tg[MiCiTnIGCipemG]9 |