RRC ID 47292
著者 Kitamura K, Kinsui EZ, Abe F.
タイトル Critical role of the proton-dependent oligopeptide transporter (POT) in the cellular uptake of the peptidyl nucleoside antibiotic, blasticidin S.
ジャーナル Biochim Biophys Acta Mol Cell Res
Abstract Blasticidin S (BlaS) interferes in the cell growth of both eukaryotes and prokaryotes. Its mode of action as a protein synthesis inhibitor has been investigated extensively. However, the mechanism of BlaS transport into the target cells is not understood well. Here, we show that Ptr2, a member of the proton-dependent oligopeptide transporter (POT) family, is responsible for the uptake of BlaS in yeasts Schizosaccharomyces pombe and Saccharomyces cerevisiae. Notably, some mutants of Ptr2 that are dysfunctional in dipeptide uptake were still competent to transport BlaS. Mouse-derived oligopeptide transporter PepT1 conferred BlaS sensitivity in the S. cerevisiae ptr2∆ mutant. Furthermore, bacterial POT family proteins also potentiated the BlaS sensitivity of E. coli. The role of the POT family oligopeptide transporters in the uptake of BlaS is conserved across species from bacteria to mammals.
巻・号 1864(2)
ページ 393-398
公開日 2017-2-1
DOI 10.1016/j.bbamcr.2016.11.030
PII S0167-4889(16)30325-1
PMID 27916534
MeSH Animals Anti-Bacterial Agents / metabolism* Membrane Transport Proteins / physiology* Mice Nucleosides / metabolism Peptide Transporter 1 Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / physiology* Symporters / physiology
IF 3.411
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
酵母 BYP7373