論文 - 詳細
RRC ID | 45415 |
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著者 | Fujioka K, Kishida T, Ejima A, Yamamoto K, Fujii W, Murakami K, Seno T, Yamamoto A, Kohno M, Oda R, Yamamoto T, Fujiwara H, Kawahito Y, Mazda O. |
タイトル | Inhibition of osteoclastogenesis by osteoblast-like cells genetically engineered to produce interleukin-10. |
ジャーナル | Biochem Biophys Res Commun |
Abstract |
Bone destruction at inflamed joints is an important complication associated with rheumatoid arthritis (RA). Interleukin-10 (IL-10) may suppress not only inflammation but also induction of osteoclasts that play key roles in the bone destruction. If IL-10-producing osteoblast-like cells are induced from patient somatic cells and transplanted back into the destructive bone lesion, such therapy may promote bone remodeling by the cooperative effects of IL-10 and osteoblasts. We transduced mouse fibroblasts with genes for IL-10 and Runx2 that is a crucial transcription factor for osteoblast differentiation. The IL-10-producing induced osteoblast-like cells (IL-10-iOBs) strongly expressed osteoblast-specific genes and massively produced bone matrix that were mineralized by calcium phosphate in vitro and in vivo. Culture supernatant of IL-10-iOBs significantly suppressed induction of osteoclast from RANKL-stimulated Raw264.7 cells as well as LPS-induced production of inflammatory cytokine by macrophages. The IL-10-iOBs may be applicable to novel cell-based therapy against bone destruction associated with RA. |
巻・号 | 456(3) |
ページ | 785-91 |
公開日 | 2015-1-16 |
DOI | 10.1016/j.bbrc.2014.12.040 |
PII | S0006-291X(14)02205-0 |
PMID | 25514036 |
MeSH | Animals Arthritis, Rheumatoid / complications Bone Matrix / immunology Bone Remodeling Bone Resorption / etiology Bone Resorption / therapy* Calcification, Physiologic Calcium Phosphates / metabolism Cell Differentiation Cell Line Cell- and Tissue-Based Therapy / methods* Core Binding Factor Alpha 1 Subunit / genetics Core Binding Factor Alpha 1 Subunit / immunology Gene Expression Regulation Genetic Engineering Interleukin-10 / genetics Interleukin-10 / immunology* Lipopolysaccharides / immunology Macrophages / immunology Mice Osteoblasts / immunology* Osteoclasts / immunology* Osteogenesis / genetics RANK Ligand / immunology Transduction, Genetic |
IF | 2.985 |
引用数 | 6 |
WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | RAW 264(RCB0535) |