RRC ID 39978
著者 Randlett O, Wee CL, Naumann EA, Nnaemeka O, Schoppik D, Fitzgerald JE, Portugues R, Lacoste AM, Riegler C, Engert F, Schier AF.
タイトル Whole-brain activity mapping onto a zebrafish brain atlas.
ジャーナル Nat Methods
Abstract In order to localize the neural circuits involved in generating behaviors, it is necessary to assign activity onto anatomical maps of the nervous system. Using brain registration across hundreds of larval zebrafish, we have built an expandable open-source atlas containing molecular labels and definitions of anatomical regions, the Z-Brain. Using this platform and immunohistochemical detection of phosphorylated extracellular signal–regulated kinase (ERK) as a readout of neural activity, we have developed a system to create and contextualize whole-brain maps of stimulus- and behavior-dependent neural activity. This mitogen-activated protein kinase (MAP)-mapping assay is technically simple, and data analysis is completely automated. Because MAP-mapping is performed on freely swimming fish, it is applicable to studies of nearly any stimulus or behavior. Here we demonstrate our high-throughput approach using pharmacological, visual and noxious stimuli, as well as hunting and feeding. The resultant maps outline hundreds of areas associated with behaviors.
巻・号 12(11)
ページ 1039-46
公開日 2015-11-1
DOI 10.1038/nmeth.3581
PMID 26778924
PMC PMC4710481
MeSH Algorithms Animals Automation Behavior, Animal Brain / metabolism* Brain / physiology Brain Mapping / methods Calcium / chemistry Extracellular Signal-Regulated MAP Kinases / metabolism* Image Processing, Computer-Assisted / methods* Immunohistochemistry Microscopy, Confocal Neurites / metabolism* Neurons / metabolism Neurons / physiology Phosphorylation Principal Component Analysis Reproducibility of Results Software Swimming Zebrafish
IF 30.822
引用数 140
WOS 分野 BIOCHEMICAL RESEARCH METHODS
リソース情報
ゼブラフィッシュ Tg(isl1:GFP)/rw0 Tg(gad1b:GFP)