RRC ID 21556
著者 Qiu X, Shimasaki Y, Tsuyama M, Yamada T, Kuwahara R, Kawaguchi M, Honda M, Gunjikake H, Tasmin R, Shimizu M, Sato Y, Kato-Unoki Y, Nakashima T, Matsubara T, Yamasaki Y, Ichinose H, Wariishi H, Honjo T, Oshima Y.
タイトル Growth-phase dependent variation in photosynthetic activity and cellular protein expression profile in the harmful raphidophyte Chattonella antiqua.
ジャーナル Biosci Biotechnol Biochem
Abstract This study investigated temporal variations in the potential maximum quantum yield of photosystem II (F(v)/F(m) ratio) and growth-phase dependent cellular protein expressions of Chattonella antiqua under laboratory conditions. Despite the culture conditions, significant positive correlations between the F(v)/F(m) ratio and daily growth rate were observed. Threshold F(v)/F(m) ratios associated with positive cell growth were calculated to be >0.44, >0.44, and >0.37, and those associated with active cell growth (growth rate >0.5 div. d(-1)) were >0.58, >0.60, and >0.49 under control culture, low nutrient and intense light conditions, respectively. Proteome profiles obtained by two-dimensional gel electrophoresis (2-DE) indicated that 42 protein spots were differentially expressed at various growth phases of C. antiqua, which indicates changes in cellular physiological status throughout the growth cycle, and suggests that oxygen evolving enhancer 1 and 2-cysteine peroxiredoxin play roles in maintaining the positive growth of C. antiqua.
巻・号 77(1)
ページ 46-52
公開日 2013-1-1
DOI 10.1271/bbb.120543
PII DN/JST.JSTAGE/bbb/120543
PMID 23291769
MeSH Algal Proteins / genetics Algal Proteins / metabolism* Amino Acid Sequence Chlorophyll / physiology Electrophoresis, Gel, Two-Dimensional Gene Expression Gene Expression Profiling Hydrogen-Ion Concentration Light Molecular Sequence Data Peroxiredoxins / genetics Peroxiredoxins / metabolism* Photosynthesis / genetics* Photosystem II Protein Complex / genetics Photosystem II Protein Complex / metabolism* Sequence Homology, Amino Acid Stramenopiles / genetics* Stramenopiles / growth & development* Stramenopiles / metabolism
IF 1.516
引用数 9
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
藻類 NIES-1