RRC ID 36379
著者 Nagata S, Kobayashi J, Kataoka H, Suzuki A.
タイトル Structural determination of an N-glycan moiety attached to the prothoracicotropic hormone from the silkmoth Bombyx mori.
ジャーナル Biosci Biotechnol Biochem
Abstract The predominant structure of the N-glycan on the prothoracicotropic hormone (PTTH) isolated from 1.8 million adult heads of silkmoths was determined to be Manα1-6Manβ1-4GlcNAcβ1-4(Fucα1-6)GlcNAc-OH, which is identical to that of the baculovirus-expressed recombinant PTTH. An ecdysis progression assay demonstrated that N-glycosylated PTTH exhibited a slightly higher activity than the recombinant PTTH without N-glycosylation produced by an Escherichia coli expression system.
巻・号 78(8)
ページ 1381-3
公開日 2014-1-1
DOI 10.1080/09168451.2014.925777
PMID 25040827
MeSH Animals Bombyx / chemistry* Carbohydrate Sequence Insect Hormones / chemistry* Molecular Sequence Data Polysaccharides / chemistry*
IF 1.516
引用数 2
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
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