RRC ID 47721
著者 Oh KH, Haney JJ, Wang X, Chuang CF, Richmond JE, Kim H.
タイトル ERG-28 controls BK channel trafficking in the ER to regulate synaptic function and alcohol response in C. elegans.
ジャーナル Elife
Abstract Voltage- and calcium-dependent BK channels regulate calcium-dependent cellular events such as neurotransmitter release by limiting calcium influx. Their plasma membrane abundance is an important factor in determining BK current and thus regulation of calcium-dependent events. In C. elegans, we show that ERG-28, an endoplasmic reticulum (ER) membrane protein, promotes the trafficking of SLO-1 BK channels from the ER to the plasma membrane by shielding them from premature degradation. In the absence of ERG-28, SLO-1 channels undergo aspartic protease DDI-1-dependent degradation, resulting in markedly reduced expression at presynaptic terminals. Loss of erg-28 suppressed phenotypic defects of slo-1 gain-of-function mutants in locomotion, neurotransmitter release, and calcium-mediated asymmetric differentiation of the AWC olfactory neuron pair, and conferred significant ethanol-resistant locomotory behavior, resembling slo-1 loss-of-function mutants, albeit to a lesser extent. Our study thus indicates that the control of BK channel trafficking is a critical regulatory mechanism for synaptic transmission and neural function.
巻・号 6
公開日 2017-2-7
DOI 10.7554/eLife.24733
PII e24733
PMID 28168949
PMC PMC5295816
MeSH Alcohols / metabolism* Animals Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / metabolism* Endoplasmic Reticulum / metabolism* Large-Conductance Calcium-Activated Potassium Channels / metabolism* Membrane Proteins / metabolism* Protein Transport Synapses / physiology*
IF 7.08
リソース情報
線虫 tm6168