RRC ID |
30204
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著者 |
Ning S, Wang N, Sakuma S, Pourkheirandish M, Wu J, Matsumoto T, Koba T, Komatsuda T.
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タイトル |
Structure, transcription and post-transcriptional regulation of the bread wheat orthologs of the barley cleistogamy gene Cly1.
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ジャーナル |
Theor Appl Genet
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Abstract |
The majority of genes present in the hexaploid bread wheat genome are present as three homoeologs. Here, we describe the three homoeologous orthologs of the barley cleistogamy gene Cly1, a member of the AP2 gene family. As in barley, the wheat genes (designated TaAP2-A, -B and -D) map to the sub-telomeric region of the long arms of the group 2 chromosomes. The structure and pattern of transcription of the TaAP2 homoeologs were similar to those of Cly1. Transcript abundance was high in the florets, and particularly in the lodicule. The TaAP2 message was cleaved at its miR172 target sites. The set of homoeolog-specific PCR assays developed will be informative for identifying either naturally occurring or induced cleistogamous alleles at each of the three wheat homoeologs. By combining such alleles via conventional crossing, it should be possible to generate a cleistogamous form of bread wheat, which would be advantageous both with respect to improving the level of the crop's resistance against the causative pathogen of fusarium head blight, and for controlling pollen-mediated gene flow to and from genetically modified cultivars.
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巻・号 |
126(5)
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ページ |
1273-83
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公開日 |
2013-5-1
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DOI |
10.1007/s00122-013-2052-6
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PMID |
23381807
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MeSH |
Alleles
Amino Acid Sequence
Base Sequence
Biomarkers / metabolism
Chromosomes, Artificial, Bacterial
Chromosomes, Plant / chemistry
Chromosomes, Plant / genetics*
Flowers / genetics*
Gene Expression Profiling
Gene Expression Regulation, Plant*
Genome, Plant
Hordeum / genetics*
Hordeum / growth & development
Hordeum / metabolism
MicroRNAs / genetics
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Phylogeny
Plant Proteins / genetics*
Plant Proteins / metabolism
RNA Processing, Post-Transcriptional
RNA, Messenger / genetics
RNA, Plant / genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Triticum / genetics*
Triticum / growth & development
Triticum / metabolism
|
IF |
4.439
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引用数 |
16
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WOS 分野
|
HORTICULTURE
AGRONOMY
PLANT SCIENCES
GENETICS & HEREDITY
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リソース情報 |
オオムギ |
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