RRC ID 4776
著者 Imai Y, Soda M, Inoue H, Hattori N, Mizuno Y, Takahashi R.
タイトル An unfolded putative transmembrane polypeptide, which can lead to endoplasmic reticulum stress, is a substrate of Parkin.
ジャーナル Cell
Abstract A putative G protein-coupled transmembrane polypeptide, named Pael receptor, was identified as an interacting protein with Parkin, a gene product responsible for autosomal recessive juvenile Parkinsonism (AR-JP). When overexpressed in cells, this receptor tends to become unfolded, insoluble, and ubiquitinated in vivo. The insoluble Pael receptor leads to unfolded protein-induced cell death. Parkin specifically ubiquitinates this receptor in the presence of ubiquitin-conjugating enzymes resident in the endoplasmic reticulum and promotes the degradation of insoluble Pael receptor, resulting in suppression of the cell death induced by Pael receptor overexpression. Moreover, the insoluble form of Pael receptor accumulates in the brains of AR-JP patients. Here, we show that the unfolded Pael receptor is a substrate of Parkin, the accumulation of which may cause selective neuronal death in AR-JP.
巻・号 105(7)
ページ 891-902
公開日 2001-6-29
DOI 10.1016/s0092-8674(01)00407-x
PII S0092-8674(01)00407-X
PMID 11439185
MeSH Acetylcysteine / analogs & derivatives* Acetylcysteine / pharmacology Brain / cytology Brain / metabolism Cell Death Cells, Cultured Cysteine Proteinase Inhibitors / pharmacology Endoplasmic Reticulum / metabolism* Humans Ligases / genetics Ligases / metabolism* Microscopy, Fluorescence Parkinsonian Disorders / genetics Parkinsonian Disorders / metabolism* Precipitin Tests Protein Binding Protein Folding* Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism* Recombinant Fusion Proteins / metabolism Two-Hybrid System Techniques Ubiquitin-Conjugating Enzymes* Ubiquitin-Protein Ligases* Ubiquitins / metabolism
IF 38.637
引用数 802
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
遺伝子材料 Human ETA receptor cDNA (RDB01302) Human ETB receptor cDNA (RDB01303) pcDNA3-hPaelR (RDB02865).