RRC ID 45156
著者 Koibuchi F, Ritoh N, Aoyagi R, Funakoshi-Tago M, Tamura H.
タイトル Dexamethasone suppresses neurosteroid biosynthesis via downregulation of steroidogenic enzyme gene expression in human glioma GI-1 cells.
ジャーナル Biol Pharm Bull
Abstract Emerging evidence indicates that stress hormone glucocorticoids (GC) are an important modulator of brain development and function. To investigate whether GCs modulate neurosteroid biosynthesis in neural cells, we studied the effects of GCs on steroidogenic gene expression in human glioma GI-1 cells. The GC dexamethasone (Dex) reduced steroidogenic acute regulatory protein (StAR), CYP11A1 and 3β-hydroxysteroid dehydrogenase gene expression in a dose- and GC receptor-dependent manner. In addition to its effects on steroidogenic gene expression, Dex also reduced de novo synthesis of progesterone (PROG). Furthermore, Dex inhibited all-trans retinoic acid (ATRA) and vitamin D3-induced steroidogenic gene expression and PROG production. This suggests that GC regulates steroidogenic gene expression in neural cells via cross-talk with the two fat-soluble vitamins, A and D. The relationship between the effects of GCs on neurosteroid biosynthesis and on cognitive behaviors and hippocampal neural activity is also discussed herein.
巻・号 37(7)
ページ 1241-7
公開日 2014-1-1
DOI 10.1248/bpb.b14-00014
PII DN/JST.JSTAGE/bpb/b14-00014
PMID 24989015
MeSH Cell Culture Techniques Cell Line, Tumor Cholesterol Side-Chain Cleavage Enzyme / genetics* Dexamethasone / pharmacology* Dose-Response Relationship, Drug Down-Regulation Gene Expression Regulation / drug effects* Glucocorticoids / pharmacology* Humans Multienzyme Complexes / genetics* Neuroglia / drug effects Neuroglia / enzymology Neuroglia / metabolism Neurotransmitter Agents / antagonists & inhibitors* Neurotransmitter Agents / biosynthesis Phosphoproteins / genetics* Progesterone / antagonists & inhibitors Progesterone / biosynthesis Progesterone Reductase / genetics* Receptors, Glucocorticoid / genetics Steroid Isomerases / genetics*
IF 1.863
引用数 0
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 GI-1(RCB0763)