| RRC ID |
47426
|
| 著者 |
Iatsenko I, Kondo S, Mengin-Lecreulx D, Lemaitre B.
|
| タイトル |
PGRP-SD, an Extracellular Pattern-Recognition Receptor, Enhances Peptidoglycan-Mediated Activation of the Drosophila Imd Pathway.
|
| ジャーナル |
Immunity
|
| Abstract |
Activation of the innate immune response in Metazoans is initiated through the recognition of microbes by host pattern-recognition receptors. In Drosophila, diaminopimelic acid (DAP)-containing peptidoglycan from Gram-negative bacteria is detected by the transmembrane receptor PGRP-LC and by the intracellular receptor PGRP-LE. Here, we show that PGRP-SD acted upstream of PGRP-LC as an extracellular receptor to enhance peptidoglycan-mediated activation of Imd signaling. Consistent with this, PGRP-SD mutants exhibited impaired activation of the Imd pathway and increased susceptibility to DAP-type bacteria. PGRP-SD enhanced the localization of peptidoglycans to the cell surface and hence promoted signaling. Moreover, PGRP-SD antagonized the action of PGRP-LB, an extracellular negative regulator, to fine-tune the intensity of the immune response. These data reveal that Drosophila PGRP-SD functions as an extracellular receptor similar to mammalian CD14 and demonstrate that, comparable to lipopolysaccharide sensing in mammals, Drosophila relies on both intra- and extracellular receptors for the detection of bacteria.
|
| 巻・号 |
45(5)
|
| ページ |
1013-1023
|
| 公開日 |
2016-11-15
|
| DOI |
10.1016/j.immuni.2016.10.029
|
| PII |
S1074-7613(16)30440-X
|
| PMID |
27851910
|
| MeSH |
Animals
Carrier Proteins / immunology*
Disease Models, Animal
Drosophila Proteins / immunology*
Drosophila melanogaster / immunology*
Gram-Negative Bacterial Infections / immunology*
Immunity, Innate / immunology
Myogenic Regulatory Factors / immunology*
Peptidoglycan / immunology
Polymerase Chain Reaction
Receptors, Pattern Recognition / immunology
Signal Transduction / immunology*
|
| IF |
22.553
|
| 引用数 |
28
|
|
WOS 分野
|
IMMUNOLOGY
|
| リソース情報 |
| ショウジョウバエ |
|
