RRC ID |
44513
|
著者 |
Kobayashi H, Motoyoshi N, Itagaki T, Tabata K, Suzuki T, Inokuchi N.
|
タイトル |
The inhibition of human tumor cell proliferation by RNase Pol, a member of the RNase T1 family, from Pleurotus ostreatus.
|
ジャーナル |
Biosci Biotechnol Biochem
|
Abstract |
RNase Po1 is a guanylic acid-specific ribonuclease (a RNase T1 family RNase) from Pleurotus ostreatus. We determined the cDNA sequence encoding RNase Po1 and expressed RNase Po1 in Escherichia coli. A comparison of the enzymatic properties of RNase Po1 and RNase T1 indicated that the optimum temperature for RNase Po1 activity was 20 °C higher than that for RNase T1. An MTT assay indicated that RNase Po1 inhibits the proliferation of human neuroblastoma cells (IMR-32 and SK-N-SH) and human leukemia cells (Jurkat and HL-60). Furthermore, Hoechst 33342 staining showed morphological changes in HL-60 cells due to RNase Po1, and flow cytometry indicated the appearance of a sub-G1 cell population. The extent of these changes was dependent on the concentration of RNase Pol. We suggest that RNase Po1 induces apoptosis in tumor cells.
|
巻・号 |
77(7)
|
ページ |
1486-91
|
公開日 |
2013-1-1
|
DOI |
10.1271/bbb.130133
|
PII |
DN/JST.JSTAGE/bbb/130133
|
PMID |
23832341
|
MeSH |
Amino Acid Sequence
Antineoplastic Agents / chemistry
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Base Sequence
Cell Cycle Checkpoints / drug effects
Cell Proliferation / drug effects
Cloning, Molecular
HL-60 Cells
Humans
Jurkat Cells
Molecular Sequence Data
Pleurotus / enzymology*
Pleurotus / genetics
Ribonuclease T1 / chemistry
Ribonuclease T1 / genetics
Ribonuclease T1 / pharmacology*
|
IF |
1.516
|
引用数 |
5
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CHEMISTRY, APPLIED
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
SK-N-SH(RCB0426) |