RRC ID 36195
著者 Nakatsukasa K, Kanada A, Matsuzaki M, Byrne SD, Okumura F, Kamura T.
タイトル The nutrient stress-induced small GTPase Rab5 contributes to the activation of vesicle trafficking and vacuolar activity.
ジャーナル J Biol Chem
Abstract Rab family small GTPases regulate membrane trafficking by spatiotemporal recruitment of various effectors. However, it remains largely unclear how the expression and functions of Rab proteins are regulated in response to extracellular or intracellular stimuli. Here we show that Ypt53, one isoform of Rab5 in Saccharomyces cerevisiae, is up-regulated significantly under nutrient stress. Under non-stress conditions, Vps21, a constitutively expressed Rab5 isoform, is crucial to Golgi-vacuole trafficking and to vacuolar hydrolase activity. However, when cells are exposed to nutrient stress for an extended period of time, the up-regulated Ypt53 and the constitutive Vps21 function redundantly to maintain these activities, which, in turn, prevent the accumulation of reactive oxygen species and maintain mitochondrial respiration. Together, our results clarify the relative roles of these constitutive and nutrient stress-inducible Rab5 proteins that ensure adaptable vesicle trafficking and vacuolar hydrolase activity, thereby allowing cells to adapt to environmental changes.
巻・号 289(30)
ページ 20970-8
公開日 2014-7-25
DOI 10.1074/jbc.M114.548297
PII S0021-9258(20)47613-6
PMID 24923442
PMC PMC4110303
MeSH Biological Transport, Active / physiology Gene Expression Regulation, Enzymologic / physiology Gene Expression Regulation, Fungal / physiology Golgi Apparatus / genetics Golgi Apparatus / metabolism Mitochondria / genetics Mitochondria / metabolism Oxygen Consumption / physiology Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / biosynthesis* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism Stress, Physiological / physiology* Up-Regulation / physiology Vacuoles / genetics Vacuoles / metabolism* rab GTP-Binding Proteins / biosynthesis* rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism rab5 GTP-Binding Proteins / biosynthesis* rab5 GTP-Binding Proteins / genetics
IF 4.238
引用数 4
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
酵母 BYP1804