RRC ID |
36645
|
著者 |
Sato Y, Nagata S, Takiguchi M.
|
タイトル |
Effective elicitation of human effector CD8+ T Cells in HLA-B*51:01 transgenic humanized mice after infection with HIV-1.
|
ジャーナル |
PLoS One
|
Abstract |
Humanized mice are expected to be useful as small animal models for in vivo studies on the pathogenesis of infectious diseases. However, it is well known that human CD8(+) T cells cannot differentiate into effector cells in immunodeficient mice transplanted with only human CD34(+) hematopoietic stem cells (HSCs), because human T cells are not educated by HLA in the mouse thymus. We here established HLA-B*51:01 transgenic humanized mice by transplanting human CD34(+) HSCs into HLA-B*51:01 transgenic NOD/SCID/Jak3(-/-) mice (hNOK/B51Tg mice) and investigated whether human effector CD8(+) T cells would be elicited in the mice or in those infected with HIV-1 NL4-3. There were no differences in the frequency of late effector memory and effector subsets (CD27(low)CD28(-)CD45RA(+/-)CCR7(-) and CD27(-)CD28(-)CD45RA(+/-)CCR7(-), respectively) among human CD8(+) T cells and in that of human CD8(+) T cells expressing CX3CR1 and/or CXCR1 between hNOK/B51Tg and hNOK mice. In contrast, the frequency of late effector memory and effector CD8(+) T cell subsets and of those expressing CX3CR1 and/or CXCR1 was significantly higher in HIV-1-infected hNOK/B51Tg mice than in uninfected ones, whereas there was no difference in that of these subsets between HIV-1-infected and uninfected hNOK mice. These results suggest that hNOK/B51Tg mice had CD8(+) T cells that were capable of differentiating into effector T cells after viral antigen stimulation and had a greater ability to elicit effector CD8(+) T cells than hNOK ones.
|
巻・号 |
7(8)
|
ページ |
e42776
|
公開日 |
2012-1-1
|
DOI |
10.1371/journal.pone.0042776
|
PII |
PONE-D-12-10664
|
PMID |
22880104
|
PMC |
PMC3412802
|
MeSH |
Animals
CD8-Positive T-Lymphocytes / immunology*
CX3C Chemokine Receptor 1
Disease Models, Animal
HIV Infections / immunology*
HIV-1 / immunology*
HLA-B51 Antigen / immunology*
Humans
Janus Kinase 3 / deficiency
Janus Kinase 3 / metabolism
Mice
Mice, Inbred NOD
Mice, SCID
Mice, Transgenic
Phenotype
Receptors, Chemokine / immunology
Receptors, Interleukin-8A / immunology
|
IF |
2.74
|
引用数 |
7
|
WOS 分野
|
IMMUNOLOGY
|
リソース情報 |
研究用ヒト臍帯血幹細胞 |
研究用ヒト臍帯血幹細胞 |