RRC ID 36662
著者 Abe T, Hanazono Y, Nagao Y.
タイトル Long-term follow-up study on the engraftment of human hematopoietic stem cells in sheep.
ジャーナル Exp Anim
Abstract Xenograft models of human hematopoiesis are essential to the study of the engraftment and proliferative potential of human hematopoietic stem cells (HSCs) in vivo. Immunodeficient mice and fetal sheep are often used as xenogeneic recipients because they are immunologically naive. In this study, we transplanted human HSCs into fetal sheep and assessed the long-term engraftment of transplanted human HSCs after birth. Fourteen sheep were used in this study. In 4 fetal sheep, HSCs were transduced with homeo-box B4 (HOXB4) gene before transplantation, which promoted the expansion of HSCs. Another 4 fetal sheep were subjected to non-myeloablative conditioning with busulfan. Seven of these 8 sheep showed successful engraftment of human HSCs (1-3% of colony-forming units) as assessed after the birth of fetal sheep (5 months post-transplantation), although HOXB4-transduced HSCs showed sustained engraftment for up to 40 months. Intact HSCs were transplanted into six non-conditioned fetal sheep, and human colony-forming units were not detected in the sheep after birth. These results suggest that, as compared with mouse models, where the short lifespan of mice limits long-term follow-up of HSC engraftment, the fetal sheep model provides a unique perspective for evaluating long-term engraftment and proliferation of human HSCs.
巻・号 63(4)
ページ 475-81
公開日 2014-1-1
DOI 10.1538/expanim.63.475
PII DN/JST.JSTAGE/expanim/14-0036
PMID 25048264
PMC PMC4244296
MeSH Animals Busulfan Cell Proliferation* Female Fetus* Follow-Up Studies Hematopoiesis* Hematopoietic Stem Cell Transplantation* Hematopoietic Stem Cells / cytology* Heterografts Homeodomain Proteins Humans Mice Models, Animal Pregnancy Sheep / embryology* Time Factors Transcription Factors Transduction, Genetic Transplantation Conditioning
IF 1.574
引用数 1
WOS 分野 VETERINARY SCIENCES ZOOLOGY
リソース情報
研究用ヒト臍帯血幹細胞 研究用ヒト臍帯血幹細胞