| RRC ID |
33205
|
| 著者 |
Danilova TV, Friebe B, Gill BS.
|
| タイトル |
Development of a wheat single gene FISH map for analyzing homoeologous relationship and chromosomal rearrangements within the Triticeae.
|
| ジャーナル |
Theor Appl Genet
|
| Abstract |
A cytogenetic map of wheat was constructed using FISH with cDNA probes. FISH markers detected homoeology and chromosomal rearrangements of wild relatives, an important source of genes for wheat improvement. To transfer agronomically important genes from wild relatives to bread wheat (Triticum aestivum L., 2n = 6 x = 42, AABBDD) by induced homoeologous recombination, it is important to know the chromosomal relationships of the species involved. Fluorescence in situ hybridization (FISH) can be used to study chromosome structure. The genomes of allohexaploid bread wheat and other species from the Triticeae tribe are colinear to some extent, i.e., composed of homoeoloci at similar positions along the chromosomes, and with genic regions being highly conserved. To develop cytogenetic markers specific for genic regions of wheat homoeologs, we selected more than 60 full-length wheat cDNAs using BLAST against mapped expressed sequence tags and used them as FISH probes. Most probes produced signals on all three homoeologous chromosomes at the expected positions. We developed a wheat physical map with several cDNA markers located on each of the 14 homoeologous chromosome arms. The FISH markers confirmed chromosome rearrangements within wheat genomes and were successfully used to study chromosome structure and homoeology in wild Triticeae species. FISH analysis detected 1 U-6 U chromosome translocation in the genome of Aegilops umbellulata, showed colinearity between chromosome A of Ae. caudata and group-1 wheat chromosomes, and between chromosome arm 7S#3 L of Thinopyrum intermedium and the long arm of the group-7 wheat chromosomes.
|
| 巻・号 |
127(3)
|
| ページ |
715-30
|
| 公開日 |
2014-3-1
|
| DOI |
10.1007/s00122-013-2253-z
|
| PMID |
24408375
|
| PMC |
PMC3931928
|
| MeSH |
Chromosome Mapping
Chromosomes, Plant / genetics*
Expressed Sequence Tags
Gene Rearrangement*
Genes, Plant
Genetic Markers
In Situ Hybridization, Fluorescence / methods*
Translocation, Genetic
Triticum / genetics*
|
| IF |
4.439
|
| 引用数 |
52
|
|
WOS 分野
|
HORTICULTURE
AGRONOMY
PLANT SCIENCES
GENETICS & HEREDITY
|
| リソース情報 |
| コムギ |
FLcDNA
KOMUGI database
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