RRC ID 37879
著者 Takagi R, Ishimaru J, Sugawara A, Toyoshima KE, Ishida K, Ogawa M, Sakakibara K, Asakawa K, Kashiwakura A, Oshima M, Minamide R, Sato A, Yoshitake T, Takeda A, Egusa H, Tsuji T.
タイトル Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model.
ジャーナル Sci Adv
Abstract The integumentary organ system is a complex system that plays important roles in waterproofing, cushioning, protecting deeper tissues, excreting waste, and thermoregulation. We developed a novel in vivo transplantation model designated as a clustering-dependent embryoid body transplantation method and generated a bioengineered three-dimensional (3D) integumentary organ system, including appendage organs such as hair follicles and sebaceous glands, from induced pluripotent stem cells. This bioengineered 3D integumentary organ system was fully functional following transplantation into nude mice and could be properly connected to surrounding host tissues, such as the epidermis, arrector pili muscles, and nerve fibers, without tumorigenesis. The bioengineered hair follicles in the 3D integumentary organ system also showed proper hair eruption and hair cycles, including the rearrangement of follicular stem cells and their niches. Potential applications of the 3D integumentary organ system include an in vitro assay system, an animal model alternative, and a bioengineered organ replacement therapy.
巻・号 2(4)
ページ e1500887
公開日 2016-4-1
DOI 10.1126/sciadv.1500887
PII 1500887
PMID 27051874
PMC PMC4820374
MeSH Animals Bioengineering* Hair Follicle / transplantation* Induced Pluripotent Stem Cells / transplantation* Integumentary System / growth & development Mice Organ Transplantation / methods Sebaceous Glands / transplantation Skin / growth & development
IF 13.117
引用数 41
リソース情報
ヒト・動物細胞 iPS-Stm-FB/gfp-99-1(APS0005) iPS-Hep-FB/Ng/gfp-103C-1(APS0007)