RRC ID 33544
著者 Serizawa M, Yamamoto H, Yamaguchi H, Fujita Y, Kobayashi K, Ogasawara N, Sekiguchi J.
タイトル Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses.
ジャーナル Gene
Abstract The SigD-regulated genes in Bacillus subtilis were systematically analyzed by comparing the pattern of transcripts derived from wild-type cells with those from sigD mutant cells using DNA microarray technology. One hundred and fifty-eight genes were found to be SigD-dependent candidates, 46 of which being known SigD-regulated genes. Northern blot analysis revealed that 18 of the remaining genes were SigD-dependent. The SigD consensus sequence was newly identified in the upstream regions of nine operons (11 genes): ybdO, yfmT-yfmS, hemAT, yjcP-yjcQ, yjfB, ylqB, yoaH, yscB and yxkC, and the other seven genes were assumed to be indirectly affected by a SigD mutation. Furthermore, yviE-yviF are likely to be SigD-dependent genes, because three independent sets of array data for yviE and yviF indicated they are SigD-dependent, and these genes are neighbors of flgL and hag transcribed by SigD RNA polymerase.
巻・号 329
ページ 125-36
公開日 2004-3-31
DOI 10.1016/j.gene.2003.12.024
PII S0378111903011661
PMID 15033535
MeSH Bacillus subtilis / genetics* Base Sequence Blotting, Northern DNA, Complementary / genetics Gene Expression Regulation, Bacterial* Genes, Bacterial / genetics* Oligonucleotide Array Sequence Analysis RNA, Messenger / genetics RNA, Messenger / metabolism Regulon / genetics Sigma Factor / metabolism* Transcription, Genetic
IF 2.984
引用数 56
WOS 分野 GENETICS & HEREDITY
リソース情報
原核生物(枯草菌) MBS61