RRC ID 33546
著者 Murayama R, Akanuma G, Makino Y, Nanamiya H, Kawamura F.
タイトル Spontaneous transformation and its use for genetic mapping in Bacillus subtilis.
ジャーナル Biosci Biotechnol Biochem
Abstract Using a simple semi-synthetic competence and sporulation medium (CSM), we found evidence that Bacillus subtilis cells transformed in the competence phase can sporulate, indicating that genetic information acquired during the competence phase is inherited by the next generation after germination of the transformed spores. Moreover, the results from mixed cell culture experiments suggest that spontaneous genetic transformation can occur between competent cells and DNA released from lysed cells in the natural environment. We also found evidence that the spontaneous transformation system can be used for genetic mapping in B. subtilis.
巻・号 68(8)
ページ 1672-80
公開日 2004-8-1
DOI 10.1271/bbb.68.1672
PII JST.JSTAGE/bbb/68.1672
PMID 15322350
MeSH Bacillus subtilis / genetics* Bacillus subtilis / growth & development Bacillus subtilis / physiology Chromosome Mapping* DNA, Bacterial / genetics* Spores / physiology* Transformation, Genetic*
IF 1.516
引用数 11
WOS 分野 CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(枯草菌) MBS419