RRC ID 35656
著者 Wiedermannová J, Sudzinová P, Kovaľ T, Rabatinová A, Šanderova H, Ramaniuk O, Rittich Š, Dohnálek J, Fu Z, Halada P, Lewis P, Krásny L.
タイトル Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis.
ジャーナル Nucleic Acids Res
Abstract Bacterial RNA polymerase (RNAP) is an essential multisubunit protein complex required for gene expression. Here, we characterize YvgS (HelD) from Bacillus subtilis, a novel binding partner of RNAP. We show that HelD interacts with RNAP-core between the secondary channel of RNAP and the alpha subunits. Importantly, we demonstrate that HelD stimulates transcription in an ATP-dependent manner by enhancing transcriptional cycling and elongation. We demonstrate that the stimulatory effect of HelD can be amplified by a small subunit of RNAP, delta. In vivo, HelD is not essential but it is required for timely adaptations of the cell to changing environment. In summary, this study establishes HelD as a valid component of the bacterial transcription machinery.
巻・号 42(8)
ページ 5151-63
公開日 2014-4-1
DOI 10.1093/nar/gku113
PII gku113
PMID 24520113
PMC PMC4005671
MeSH Adenosine Triphosphate / metabolism Bacillus subtilis / enzymology* Bacillus subtilis / genetics Bacterial Proteins / isolation & purification Bacterial Proteins / metabolism* DNA / metabolism DNA-Directed RNA Polymerases / chemistry DNA-Directed RNA Polymerases / isolation & purification DNA-Directed RNA Polymerases / metabolism* Phenotype Transcription Elongation, Genetic Transcription, Genetic*
IF 11.502
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(枯草菌)