RRC ID 41215
著者 Chen S, Liu HC, Zhao D, Yang J, Zhou J, Xiang H.
タイトル Halorubrum yunnanense sp. nov., isolated from a subterranean salt mine.
ジャーナル Int J Syst Evol Microbiol
Abstract Two halophilic archaeal strains, Q85(T) and Q86, were isolated from a subterranean salt mine in Yunnan, China. Cells were rod-shaped, Gram-stain-negative and motile. Colonies were red, smooth, convex and round (1.0-2.0 mm in diameter). The orthologous 16S rRNA and rpoB9 gene sequences of these two strains were almost identical (99.5 and 99.7 % similarities). Their closest relatives were Halorubrum kocurii BG-1(T) (98.0-98.1 % 16S rRNA gene sequence similarity), Halorubrum aidingense 31-hong(T) (97.6–97.7 %) and Halorubrum lipolyticum 9-3T (97.5-97.6 %). The level of DNA-DNA relatedness between strains Q85(T) and Q86 was 90 %, while that between Q85(T) and other related Halorubrum strains was less than 30 % (29 % for H. kocurii BG-1(T), 25 % for H. aidingense 31-hong(T) and 22 % for H. lipolyticum 9-3(T)). Optimal growth of the two novel strains was observed with 20 % (w/v) NaCl and at 42–45 8C under aerobic conditions, with a slight difference in optimum Mg2+ concentration (0.7 M for Q85(T),0.5 M for Q86) and a notable difference in optimum pH (pH 7.5 for Q85(T), pH 6.6 for Q86). Anaerobic growth occurred with nitrate, but not with L-arginine or DMSO. The major polar lipids of the two strains were identical, including phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and sulfated diglycosyl diether, which are the major lipids of the genus Halorubrum. The G+C contents of strains Q85(T) and Q86 were 66.3 and 66.8 %, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic properties of strains Q85(T) and Q86, a novel species, Halorubrum yunnanense sp. nov., is proposed. The type strain is Q85(T) (=5CGMCC 1.15057(T)=JCM 30665(T)).
巻・号 65(12)
ページ 4526-4532
公開日 2015-12-1
DOI 10.1099/ijsem.0.000605
PMID 26374710
MeSH Base Composition China DNA, Archaeal / genetics Fatty Acids / genetics Genes, Archaeal Halorubrum / classification* Halorubrum / genetics Halorubrum / isolation & purification Mining* Molecular Sequence Data Nucleic Acid Hybridization Phosphatidylglycerols / chemistry Phospholipids / chemistry Phylogeny* Pigmentation RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride*
IF 2.415
引用数 7
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 30665 JCM 30666