RRC ID 35502
著者 Galli E, Gerdes K.
タイトル FtsZ-ZapA-ZapB interactome of Escherichia coli.
ジャーナル J Bacteriol
Abstract Bacterial cell division relies on the formation and contraction of the Z ring, coordinated and regulated by a dynamic protein complex called the divisome. The cell division factor ZapA interacts directly with FtsZ and thereby increases FtsZ protofilament association and Z-ring stability. Here, we investigated ZapB interaction with ZapA and its effect on Z-ring formation and FtsZ protofilament bundling. The combination of the ftsZ84 allele that encodes an FtsZ variant that polymerizes inefficiently with a zapB null mutant resulted in a synthetic defective phenotype. Overproduction of ZapA led to the formation of aberrant FtsZ helical structures and delocalization of ZapB. The N-terminal end of ZapB was essential for ZapB-ZapA interaction, and amino acid changes close to the N terminus of ZapB exhibited reduced interaction with ZapA. Sedimentation assays showed that ZapB interacts strongly with ZapA and reduces ZapA's interaction with FtsZ in vitro. The morphology of the structures formed by ZapA and ZapB together was similar to the cables formed by ZapB in the presence of CaCl(2), a known ZapB bundling agent. The in vivo and in vitro data support a model in which ZapA interacts strongly with ZapB and the ZapA-ZapB interaction is favored over ZapA-FtsZ.
巻・号 194(2)
ページ 292-302
公開日 2012-1-1
DOI 10.1128/JB.05821-11
PII JB.05821-11
PMID 22056926
PMC PMC3256659
MeSH Bacterial Proteins / genetics Bacterial Proteins / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cytokinesis Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism* Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial / physiology* Green Fluorescent Proteins Hydrogen-Ion Concentration Mutation Plasmids Time Factors
IF 3.006
引用数 53
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌)