RRC ID 45704
著者 Chen S, Li L, Li J, Liu B, Zhu X, Zheng L, Zhang R, Xu T.
タイトル SEC-10 and RAB-10 coordinate basolateral recycling of clathrin-independent cargo through endosomal tubules in Caenorhabditis elegans.
ジャーナル Proc Natl Acad Sci U S A
Abstract Despite the increasing number of regulatory proteins identified in clathrin-independent endocytic (CIE) pathways, our understanding of the exact functions of these proteins and the sequential manner in which they function remains limited. In this study, using the Caenorhabditis elegans intestine as a model, we observed a unique structure of interconnected endosomal tubules, which is required for the basolateral recycling of several CIE cargoes including hTAC, GLUT1, and DAF-4. SEC-10 is a subunit of the octameric protein complex exocyst. Depleting SEC-10 and several other exocyst components disrupted the endosomal tubules into various ring-like structures. An epistasis analysis further suggested that SEC-10 operates at the intermediate step between early endosomes and recycling endosomes. The endosomal tubules were also sensitive to inactivation of the Rab GTPase RAB-10 and disruption of microtubules. Taken together, our data suggest that SEC-10 coordinates with RAB-10 and microtubules to form the endosomal tubular network for efficient recycling of particular CIE cargoes.
巻・号 111(43)
ページ 15432-7
公開日 2014-10-28
DOI 10.1073/pnas.1408327111
PII 1408327111
PMID 25301900
PMC PMC4217428
MeSH Animals Biological Transport Caenorhabditis elegans / cytology* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Clathrin / metabolism* Endocytosis* Endosomes / metabolism* Humans Intestinal Mucosa / metabolism Intestines / cytology Lysosomes / metabolism Membrane Fusion Microtubules / metabolism Mutation / genetics Vesicular Transport Proteins rab GTP-Binding Proteins / metabolism*
IF 9.412
引用数 18
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm1443