RRC ID |
36156
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著者 |
Sumitani M, Sakurai T, Kasashima K, Kobayashi S, Uchino K, Kanzaki R, Tamura T, Sezutsu H.
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タイトル |
Establishment of a specific cell death induction system in Bombyx mori by a transgene with the conserved apoptotic regulator, mouse Bcl-2-associated X protein (mouse Bax).
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ジャーナル |
Insect Mol Biol
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Abstract |
The induction of apoptosis in vivo is a useful tool for investigating the functions and importance of particular tissues. B-cell leukaemia/lymphoma 2-associated X protein (Bax) functions as a pro-apoptotic factor and induces apoptosis in several organisms. The Bax-mediated apoptotic system is widely conserved from Caenorhabditis elegans to humans. In order to establish a tissue-specific cell death system in the domestic silkworm, Bombyx mori, we constructed a transgenic silkworm that overexpressed mouse Bax (mBax) in particular tissues by the Gal4-upstream activation sequence system. We found that the expression of mBax induced specific cell death in the silk gland, fat body and sensory cells. Fragmentation of genomic DNA was observed in the fat body, which expressed mBax, thereby supporting apoptotic cell death in this tissue. Using this system, we also demonstrated that specific cell death in sensory cells attenuated the response to the sex pheromone bombykol. These results show that we successfully established a tissue-specific cell death system in vivo that enabled specific deficiencies in particular tissues. The inducible cell death system may provide useful means for industrial applications of the silkworm and possible utilization for other species.
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巻・号 |
24(6)
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ページ |
671-80
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公開日 |
2015-12-1
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DOI |
10.1111/imb.12192
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PMID |
26426866
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MeSH |
Animals
Animals, Genetically Modified
Apoptosis
Bombyx / cytology
Bombyx / genetics*
Bombyx / growth & development
Exocrine Glands / physiology
Fat Body / metabolism
Fatty Alcohols / pharmacology
Female
Larva / growth & development
Male
Mice
Olfactory Receptor Neurons / cytology
Olfactory Receptor Neurons / drug effects
Receptors, Odorant / genetics
Receptors, Odorant / metabolism
Sex Attractants / pharmacology
Transgenes
bcl-2-Associated X Protein / genetics*
|
IF |
2.533
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引用数 |
7
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WOS 分野
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
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リソース情報 |
カイコ |
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