RRC ID 36156
著者 Sumitani M, Sakurai T, Kasashima K, Kobayashi S, Uchino K, Kanzaki R, Tamura T, Sezutsu H.
タイトル Establishment of a specific cell death induction system in Bombyx mori by a transgene with the conserved apoptotic regulator, mouse Bcl-2-associated X protein (mouse Bax).
ジャーナル Insect Mol Biol
Abstract The induction of apoptosis in vivo is a useful tool for investigating the functions and importance of particular tissues. B-cell leukaemia/lymphoma 2-associated X protein (Bax) functions as a pro-apoptotic factor and induces apoptosis in several organisms. The Bax-mediated apoptotic system is widely conserved from Caenorhabditis elegans to humans. In order to establish a tissue-specific cell death system in the domestic silkworm, Bombyx mori, we constructed a transgenic silkworm that overexpressed mouse Bax (mBax) in particular tissues by the Gal4-upstream activation sequence system. We found that the expression of mBax induced specific cell death in the silk gland, fat body and sensory cells. Fragmentation of genomic DNA was observed in the fat body, which expressed mBax, thereby supporting apoptotic cell death in this tissue. Using this system, we also demonstrated that specific cell death in sensory cells attenuated the response to the sex pheromone bombykol. These results show that we successfully established a tissue-specific cell death system in vivo that enabled specific deficiencies in particular tissues. The inducible cell death system may provide useful means for industrial applications of the silkworm and possible utilization for other species.
巻・号 24(6)
ページ 671-80
公開日 2015-12-1
DOI 10.1111/imb.12192
PMID 26426866
MeSH Animals Animals, Genetically Modified Apoptosis Bombyx / cytology Bombyx / genetics* Bombyx / growth & development Exocrine Glands / physiology Fat Body / metabolism Fatty Alcohols / pharmacology Female Larva / growth & development Male Mice Olfactory Receptor Neurons / cytology Olfactory Receptor Neurons / drug effects Receptors, Odorant / genetics Receptors, Odorant / metabolism Sex Attractants / pharmacology Transgenes bcl-2-Associated X Protein / genetics*
IF 2.533
引用数 7
WOS 分野 ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
カイコ