RRC ID 36420
著者 Tan D, Tong XL, Hu H, Wu SY, Li CL, Xiong G, Xiang ZH, Dai FY, Lu C.
タイトル Morphological characterization and molecular mapping of an irradiation-induced Speckled mutant in the silkworm, Bombyx mori.
ジャーナル Insect Mol Biol
Abstract Speckled (Spc), an X-ray-induced lethal mutant of Bombyx mori, exhibits a mosaic dark-brown-spotted larval epidermis in both sexes and egg-laying problems only in females. Here, we report the morphological characterization and molecular mapping of the Spc mutant. Morphological investigations revealed that the epidermal ultrastructure of the small, dark-brown spots was more dense than that of the white regions in both Spc/+ mutants and wild type, and that the lethality of the Spc/Spc mutants occurred during early embryogenesis. Furthermore, the ovarioles and ovipositor were disconnected in approximately 85.5% of Spc/+ females, a further 2.5% had a connection between the ovarioles and ovipositor that was too narrow to lay eggs. The remaining females showed a normal connection similar to that of the wild type. We successfully narrowed down the location of the Spc mutation to a region on chromosome 4 that was ∼1041 kb long. Gene-prediction analysis identified 25 candidate genes in this region. Chromosome structure analysis indicated that a ∼305 kb deletion was included in the mapping region. Temporal and spatial reverse transcription PCR (RT-PCR) analysis showed that several genes in the mapped region are associated with the Spc mutant. Although the genes responsible for the Spc mutation were not definitively identified, our results further the current understanding of the complex mechanism underlying the multiple morphological defects in Spc mutants.
巻・号 25(2)
ページ 93-104
公開日 2016-4-1
DOI 10.1111/imb.12205
PMID 26661290
MeSH Animals Bombyx / genetics* Bombyx / growth & development Bombyx / radiation effects Chromosome Mapping Embryonic Development / genetics* Embryonic Development / radiation effects Epidermis / growth & development Female Larva / genetics* Larva / growth & development Larva / radiation effects Male Mutation / radiation effects Pigmentation / genetics* X-Rays
IF 2.533
引用数 3
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY ENTOMOLOGY
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