RRC ID 47683
著者 Xiao Q, Zhang C, Li H, Wei B, Wang Y, Huang H, Li Y, Yu G, Liu H, Zhang J, Liu Y, Hu Y, Huang Y.
タイトル Identification and functional analysis of the ICK gene family in maize.
ジャーナル Sci Rep
Abstract Inhibitors of cyclin-dependent kinases (ICKs) are key regulators of cyclin-dependent kinase activities and cell division. Herein, we identified eight ICKs in maize, which we named Zeama;ICKs (ZmICKs). Primary sequencing and phylogenetic analyses were used to divide the ZmICK family into two classes: group B and group C. Subcellular localization analysis of ZmICK:enhanced green fluorescent protein (eGFP) fusion constructs in tobacco leaf cells indicated that ZmICKs are principally nuclear. Co-localization analysis of the ZmICKs and maize A-type cyclin-dependent kinase (ZmCDKA) was also performed using enhanced green fluorescent protein (eGFP) and red fluorescent protein (RFP) fusion constructs. The ZmICKs and ZmCDKA co-localized in the nucleus. Semi-quantitative RT-PCR analysis of the ZmICKs showed that they were expressed at different levels in all tissues examined and shared similar expression patterns with cell cycle-related genes. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that ZmICK1, ZmICK2, ZmICK3, and ZmICK4 interact with ZmCDKA1 and ZmCDKA3. Interestingly, ZmICK7 interacts with D-type cyclins. Transformed and expressed ZmCDKA1 and ZmICKs together in fission yeast revealed that ZmICK1, ZmICK3, and ZmICK4 can affect ZmCDKA1 function. Moreover, the C-group of ZmICKs could interact with ZmCDKA1 directly and affect ZmCDKA1 function, suggesting that C-group ZmICKs are important for cell division regulation.
巻・号 7
ページ 43818
公開日 2017-3-6
DOI 10.1038/srep43818
PII srep43818
PMID 28262730
PMC PMC5338338
MeSH Cell Cycle / genetics Cell Division / genetics Cell Nucleus / metabolism Cyclin-Dependent Kinase Inhibitor Proteins / chemistry Cyclin-Dependent Kinase Inhibitor Proteins / genetics* Cyclin-Dependent Kinase Inhibitor Proteins / metabolism Gene Expression Regulation, Plant* Luminescent Proteins / genetics Luminescent Proteins / metabolism Multigene Family* Phylogeny Plant Proteins / classification Plant Proteins / genetics* Plant Proteins / metabolism Plants, Genetically Modified Protein Binding Zea mays / genetics* Zea mays / metabolism
IF 3.998
引用数 3
WOS 分野 PLANT SCIENCES
リソース情報
酵母 FY12137