RRC ID |
5682
|
著者 |
Lu R, Yigit E, Li WX, Ding SW.
|
タイトル |
An RIG-I-Like RNA helicase mediates antiviral RNAi downstream of viral siRNA biogenesis in Caenorhabditis elegans.
|
ジャーナル |
PLoS Pathog
|
Abstract |
Dicer ribonucleases of plants and invertebrate animals including Caenorhabditis elegans recognize and process a viral RNA trigger into virus-derived small interfering RNAs (siRNAs) to guide specific viral immunity by Argonaute-dependent RNA interference (RNAi). C. elegans also encodes three Dicer-related helicase (drh) genes closely related to the RIG-I-like RNA helicase receptors which initiate broad-spectrum innate immunity against RNA viruses in mammals. Here we developed a transgenic C. elegans strain that expressed intense green fluorescence from a chromosomally integrated flock house virus replicon only after knockdown or knockout of a gene required for antiviral RNAi. Use of the reporter nematode strain in a feeding RNAi screen identified drh-1 as an essential component of the antiviral RNAi pathway. However, RNAi induced by either exogenous dsRNA or the viral replicon was enhanced in drh-2 mutant nematodes, whereas exogenous RNAi was essentially unaltered in drh-1 mutant nematodes, indicating that exogenous and antiviral RNAi pathways are genetically distinct. Genetic epistatic analysis shows that drh-1 acts downstream of virus sensing and viral siRNA biogenesis to mediate specific antiviral RNAi. Notably, we found that two members of the substantially expanded subfamily of Argonautes specific to C. elegans control parallel antiviral RNAi pathways. These findings demonstrate both conserved and unique strategies of C. elegans in antiviral defense.
|
巻・号 |
5(2)
|
ページ |
e1000286
|
公開日 |
2009-2-1
|
DOI |
10.1371/journal.ppat.1000286
|
PMID |
19197349
|
PMC |
PMC2629121
|
MeSH |
Animals
Animals, Genetically Modified
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / immunology
Caenorhabditis elegans / metabolism
Caenorhabditis elegans / virology*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism
Caenorhabditis elegans Proteins / physiology*
DEAD-box RNA Helicases / genetics
DEAD-box RNA Helicases / physiology*
Epistasis, Genetic
Gene Expression Regulation
Gene Targeting
Genes, Helminth
Microscopy, Fluorescence
Mutation
Nodaviridae / genetics
RNA Interference*
RNA, Small Interfering / genetics
RNA, Small Interfering / metabolism*
RNA, Viral / metabolism
Replicon
|
IF |
6.218
|
引用数 |
78
|
WOS 分野
|
PARASITOLOGY
MICROBIOLOGY
VIROLOGY
|
リソース情報 |
線虫 |
tm1329
tm1217
tm1860
tm1637 |