RRC ID 45678
著者 Armenti ST, Chan E, Nance J.
タイトル Polarized exocyst-mediated vesicle fusion directs intracellular lumenogenesis within the C. elegans excretory cell.
ジャーナル Dev Biol
Abstract Lumenogenesis of small seamless tubes occurs through intracellular membrane growth and directed vesicle fusion events. Within the Caenorhabditis elegans excretory cell, which forms seamless intracellular tubes (canals) that mediate osmoregulation, lumens grow in length and diameter when vesicles fuse with the expanding lumenal surface. Here, we show that lumenal vesicle fusion depends on the small GTPase RAL-1, which localizes to vesicles and acts through the exocyst vesicle-tethering complex. Loss of either the exocyst or RAL-1 prevents excretory canal lumen extension. Within the excretory canal and other polarized cells, the exocyst co-localizes with the PAR polarity proteins PAR-3, PAR-6 and PKC-3. Using early embryonic cells to determine the functional relationships between the exocyst and PAR proteins, we show that RAL-1 recruits the exocyst to the membrane, while PAR proteins concentrate membrane-localized exocyst proteins to a polarized domain. These findings reveal that RAL-1 and the exocyst direct the polarized vesicle fusion events required for intracellular lumenogenesis of the excretory cell, suggesting mechanistic similarities in the formation of topologically distinct multicellular and intracellular lumens.
巻・号 394(1)
ページ 110-21
公開日 2014-10-1
DOI 10.1016/j.ydbio.2014.07.019
PII S0012-1606(14)00355-8
PMID 25102190
PMC PMC4373406
MeSH Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Membrane Fusion / physiology* Osmoregulation / genetics Protein Kinase C / genetics Protein Kinase C / metabolism Protein Serine-Threonine Kinases RNA Interference RNA, Small Interfering Secretory Vesicles / metabolism* Vesicular Transport Proteins / genetics ral GTP-Binding Proteins / genetics*
IF 2.896
引用数 28
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm5205