RRC ID 47476
著者 Kishimoto K, Nakayama M, Kinoshita M.
タイトル In vivo recombination efficiency of two site-specific recombination systems, VCre/VloxP and SCre/SloxP, in medaka (Oryzias latipes).
ジャーナル Dev Growth Differ
Abstract The present study delineates the in vivo efficiency of two site-specific recombination systems, VCre/VloxP and SCre/SloxP, in medaka (Oryzias latipes). VCre, SCre, and Cre RNA was microinjected into fertilized medaka eggs belonging to three transgenic lines harboring VloxP, SloxP, and loxP cassette. VCre induced site-specific recombination specifically at VloxP sequence and SCre at SloxP sequence without any cross-reactivity. These findings provide two novel alternative recombination systems in vivo in addition to the existing Cre/loxP and Flp/FRT systems, thus enabling sophisticated gene expression in model organisms.
巻・号 58(6)
ページ 516-21
公開日 2016-8-1
DOI 10.1111/dgd.12289
PMID 27224047
MeSH Animals Female Gene Expression Regulation* Genetic Engineering / methods* Integrases* Male Oryzias* / genetics Oryzias* / metabolism Recombination, Genetic*
IF 1.723
引用数 2
WOS 分野 CELL BIOLOGY DEVELOPMENTAL BIOLOGY
リソース情報
遺伝子材料 pactb-loxP-mCherry-loxP-EGFP (RDB14532) pCS2-OlCre (RDB14533).