RRC ID 34808
著者 Zou W, Yadav S, DeVault L, Nung Jan Y, Sherwood DR.
タイトル RAB-10-Dependent Membrane Transport Is Required for Dendrite Arborization.
ジャーナル PLoS Genet
Abstract Formation of elaborately branched dendrites is necessary for the proper input and connectivity of many sensory neurons. Previous studies have revealed that dendritic growth relies heavily on ER-to-Golgi transport, Golgi outposts and endocytic recycling. How new membrane and associated cargo is delivered from the secretory and endosomal compartments to sites of active dendritic growth, however, remains unknown. Using a candidate-based genetic screen in C. elegans, we have identified the small GTPase RAB-10 as a key regulator of membrane trafficking during dendrite morphogenesis. Loss of rab-10 severely reduced proximal dendritic arborization in the multi-dendritic PVD neuron. RAB-10 acts cell-autonomously in the PVD neuron and localizes to the Golgi and early endosomes. Loss of function mutations of the exocyst complex components exoc-8 and sec-8, which regulate tethering, docking and fusion of transport vesicles at the plasma membrane, also caused proximal dendritic arborization defects and led to the accumulation of intracellular RAB-10 vesicles. In rab-10 and exoc-8 mutants, the trans-membrane proteins DMA-1 and HPO-30, which promote PVD dendrite stabilization and branching, no longer localized strongly to the proximal dendritic membranes and instead were sequestered within intracellular vesicles. Together these results suggest a crucial role for the Rab10 GTPase and the exocyst complex in controlling membrane transport from the secretory and/or endosomal compartments that is required for dendritic growth.
巻・号 11(9)
ページ e1005484
公開日 2015-1-1
DOI 10.1371/journal.pgen.1005484
PII PGENETICS-D-15-00575
PMID 26394140
PMC PMC4578882
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Dendrites / genetics* Dendrites / physiology Endocytosis / genetics Endoplasmic Reticulum / genetics Endosomes / genetics Golgi Apparatus / genetics Membrane Proteins / genetics Membrane Transport Modulators / metabolism Neuronal Plasticity / genetics* Sensory Receptor Cells / metabolism* rab GTP-Binding Proteins / genetics* rab GTP-Binding Proteins / metabolism
IF 5.175
引用数 29
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 dma-1(tm5159) rab-8 (tm2526) sec-5 (tm1413) sec-10 (tm3437) sec-5 (tm1443)