RRC ID 47328
著者 Carr D, Sanchez-Alvarez L, Imai JH, Slatculescu C, Noblett N, Mao L, Beese L, Colavita A.
タイトル A Farnesyltransferase Acts to Inhibit Ectopic Neurite Formation in C. elegans.
ジャーナル PLoS One
Abstract Genetic pathways that regulate nascent neurite formation play a critical role in neuronal morphogenesis. The core planar cell polarity components VANG-1/Van Gogh and PRKL-1/Prickle are involved in blocking inappropriate neurite formation in a subset of motor neurons in C. elegans. A genetic screen for mutants that display supernumerary neurites was performed to identify additional factors involved in this process. This screen identified mutations in fntb-1, the β subunit of farnesyltransferase. We show that fntb-1 is expressed in neurons and acts cell-autonomously to regulate neurite formation. Prickle proteins are known to be post-translationally modified by farnesylation at their C-terminal CAAX motifs. We show that PRKL-1 can be recruited to the plasma membrane in both a CAAX-dependent and CAAX-independent manner but that PRKL-1 can only inhibit neurite formation in a CAAX-dependent manner.
巻・号 11(6)
ページ e0157537
公開日 2016-1-1
DOI 10.1371/journal.pone.0157537
PII PONE-D-16-03536
PMID 27300162
PMC PMC4907426
MeSH Animals Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Farnesyltranstransferase / analysis Farnesyltranstransferase / genetics Farnesyltranstransferase / metabolism* Intracellular Signaling Peptides and Proteins / analysis Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism* Models, Molecular Mutation Neurites / physiology* Prenylation Protein Processing, Post-Translational Protein Subunits / analysis Protein Subunits / genetics Protein Subunits / metabolism
IF 2.74
引用数 1
WOS 分野 MULTIDISCIPLINARY SCIENCES
リソース情報
線虫 vang-1(tm1422)