| RRC ID |
45632
|
| 著者 |
Reid A, Sherry TJ, Yücel D, Llamosas E, Nicholas HR.
|
| タイトル |
The C-terminal binding protein (CTBP-1) regulates dorsal SMD axonal morphology in Caenorhabditis elegans.
|
| ジャーナル |
Neuroscience
|
| Abstract |
C-terminal binding proteins (CtBPs) are transcriptional co-repressors which cooperate with a variety of transcription factors to repress gene expression. Caenorhabditis elegans CTBP-1 expression has been observed in the nervous system and hypodermis. In C. elegans, CTBP-1 regulates several processes including Acute Functional Tolerance to ethanol and functions in the nervous system to modulate both lifespan and expression of a lipase gene called lips-7. Incorrect structure and/or function of the nervous system can lead to behavioral changes. Here, we demonstrate reduced exploration behavior in ctbp-1 mutants. Our examination of a subset of neurons involved in regulating locomotion revealed that the axonal morphology of dorsal SMD (SMDD) neurons is altered in ctbp-1 mutants at the fourth larval (L4) stage. Expressing CTBP-1 under the control of the endogenous ctbp-1 promoter rescued both the exploration behavior phenotype and defective SMDD axon structure in ctbp-1 mutants at the L4 stage. Interestingly, the pre-synaptic marker RAB-3 was found to localize to the mispositioned portion of SMDD axons in a ctbp-1 mutant. Further analysis of SMDD axonal morphology at days 1, 3 and 5 of adulthood revealed that the number of ctbp-1 mutants showing an SMDD axonal morphology defect increases in early adulthood and the observed defect appears to be qualitatively more severe. CTBP-1 is prominently expressed in the nervous system with weak expression detected in the hypodermis. Surprisingly, solely expressing CTBP-1a in the nervous system or hypodermis did not restore correct SMDD axonal structure in a ctbp-1 mutant. Our results demonstrate a role for CTBP-1 in exploration behavior and the regulation of SMDD axonal morphology in C. elegans.
|
| 巻・号 |
311
|
| ページ |
216-30
|
| 公開日 |
2015-12-17
|
| DOI |
10.1016/j.neuroscience.2015.10.026
|
| PII |
S0306-4522(15)00933-1
|
| PMID |
26480814
|
| MeSH |
Animals
Animals, Genetically Modified
Axons / metabolism*
Caenorhabditis elegans / cytology*
Caenorhabditis elegans / growth & development
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Exploratory Behavior / physiology
Microscopy, Fluorescence
Mutation
Repressor Proteins / genetics
Repressor Proteins / metabolism*
rab3 GTP-Binding Proteins / metabolism
|
| IF |
3.056
|
| 引用数 |
2
|
|
WOS 分野
|
NEUROSCIENCES
|
| リソース情報 |
| 線虫 |
tm5512 |
