RRC ID 45668
著者 Zheng C, Jin FQ, Chalfie M.
タイトル Hox Proteins Act as Transcriptional Guarantors to Ensure Terminal Differentiation.
ジャーナル Cell Rep
Abstract Cell differentiation usually occurs with high fidelity, but the expression of many transcription factors is variable. Using the touch receptor neurons (TRNs) in C. elegans, we found that the Hox proteins CEH-13/lab and EGL-5/Abd-B overcome this variability by facilitating the activation of the common TRN fate determinant mec-3 in the anterior and posterior TRNs, respectively. CEH-13 and EGL-5 increase the probability of mec-3 transcriptional activation by the POU-homeodomain transcription factor UNC-86 using the same Hox/Pbx binding site. Mutation of ceh-13 and egl-5 resulted in an incomplete (∼40%) loss of the TRN fate in respective TRNs, which correlates with quantitative mRNA measurements showing two distinct modes (all or none) of mec-3 transcription. Therefore, Hox proteins act as transcriptional "guarantors" in order to ensure reliable and robust gene expression during terminal neuronal differentiation. Guarantors do not activate gene expression by themselves but promote full activation of target genes regulated by other transcription factors.
巻・号 13(7)
ページ 1343-1352
公開日 2015-11-17
DOI 10.1016/j.celrep.2015.10.044
PII S2211-1247(15)01214-0
PMID 26547238
PMC PMC4654970
MeSH Animals Base Sequence Binding Sites Caenorhabditis elegans Caenorhabditis elegans Proteins / physiology* Gene Expression Regulation, Developmental Homeodomain Proteins / physiology* Neurogenesis* Neurons / metabolism Promoter Regions, Genetic Transcription, Genetic
IF 8.109
引用数 13
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm4746