RRC ID 34049
著者 Sugawara A, Matsui D, Komeda H, Asano Y, Isobe K.
タイトル Characterization and application of aminoamide-oxidizing enzyme from Aspergillus carbonarius AIU 205.
ジャーナル J Biosci Bioeng
Abstract We isolated Aspergillus carbonarius AIU 205 as a new producer of an enzyme catalyzing oxidative deamination of 4-aminobutanamide (4-ABAD) to 4-oxobutanamide with the subsequent release of ammonia and hydrogen peroxide. Since the strain produced three enzymes with different Km values for 4-ABAD, the enzyme with lowest Km value (0.31 mM) was purified and revealed certain remarkable properties. The enzyme also oxidized aliphatic monoamines, aromatic amines and aliphatic aminoalcohols, but did not oxidize l-amino acids and aliphatic diamines. The Vmax/Km values for aliphatic monoamines were higher than that for 4-ABAD, and the enzyme activity was strongly inhibited by inhibitors of copper-containing amine oxidases. Thus, it was concluded that the enzyme might belong to a group of copper-containing amine oxidase. The 4-ABAD oxidase activity of this enzyme was optimum at pH 7.0, and the enzyme activity at pH 6.0 was 65% of that at pH 7.0. The enzyme was useful for increasing the sensitivity of l-lysine assay using l-amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813.
巻・号 117(3)
ページ 263-8
公開日 2014-3-1
DOI 10.1016/j.jbiosc.2013.08.019
PII S1389-1723(13)00320-4
PMID 24113361
MeSH Amides / metabolism* Amine Oxidase (Copper-Containing) / isolation & purification Amine Oxidase (Copper-Containing) / metabolism* Aspergillus / enzymology* Hydrogen-Ion Concentration L-Amino Acid Oxidase / isolation & purification L-Amino Acid Oxidase / metabolism Lysine / metabolism Oxidation-Reduction Pseudomonas / enzymology Substrate Specificity Temperature
IF 2.366
引用数 4
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
一般微生物 JCM 5546 JCM 5548 JCM 5697