RRC ID 44642
著者 Yamazoe H, Sugiyama Y, El Omri A, Hagihara Y, Okada T.
タイトル Facile immunostaining and labeling of nonadherent cells using a microfluidic device to entrap the cells.
ジャーナル J Biosci Bioeng
Abstract We fabricated a microfluidic device for the entrapment of nonadherent cells. Solution exchange was easily performed by introducing the solution into the cell-trapping microchannel. Immunostaining and labeling of the cell membrane of THP-1 cells were demonstrated using this device, which does not require cumbersome repetition of centrifugation and resuspension steps.
巻・号 117(3)
ページ 375-8
公開日 2014-3-1
DOI 10.1016/j.jbiosc.2013.08.008
PII S1389-1723(13)00309-5
PMID 24035233
MeSH Actins / metabolism Cell Adhesion Cell Line Cell Membrane / metabolism* Cell Separation / instrumentation Cell Separation / methods* Fluorescent Dyes Humans Microfluidic Analytical Techniques / instrumentation* Monocytes / cytology Monocytes / immunology Monocytes / metabolism
IF 2.366
引用数 3
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
ヒト・動物細胞 THP-1(RCB1189)