RRC ID 30493
著者 Ono H.
タイトル Ecdysone differentially regulates metamorphic timing relative to 20-hydroxyecdysone by antagonizing juvenile hormone in Drosophila melanogaster.
ジャーナル Dev Biol
Abstract In insects, a steroid hormone, 20-hydroxyecdysone (20E), plays important roles in the regulation of developmental transitions by initiating signaling cascades via the ecdysone receptor (EcR). Although 20E has been well characterized as the molting hormone, its precursor ecdysone (E) has been considered to be a relatively inactive compound because it has little or no effect on classic EcR mediated responses. I found that feeding E to wild-type third instar larvae of Drosophila melanogaster accelerates the metamorphic timing, which results in elevation of lethality during metamorphosis and reduced body size, while 20E has only a minor effect. The addition of a juvenile hormone analog (JHA) to E impeded their precocious pupariation and thereby rescued the reduced body size. The ability of JHA impeding the effect of E was not observed in the Methoprene-tolerant (Met) and germ-cell expressed (gce) double mutant animals lacking JH signaling, indicating that antagonistic action of JH against E is transduced via a primary JH receptor, Met, or a product of its homolog, Gce. I also found that L3 larvae are susceptible to E around the time when they reach their minimum viable weight. These results indicate that E, and not just 20E, is also essential for proper regulation of developmental timing and body size. Furthermore, the precocious pupariation triggered by E is impeded by the action of JH to ensure that animals attain body size to survive metamorphosis.
巻・号 391(1)
ページ 32-42
公開日 2014-7-1
DOI 10.1016/j.ydbio.2014.04.004
PII S0012-1606(14)00196-1
PMID 24727669
MeSH Animals Animals, Genetically Modified Body Size / physiology Dose-Response Relationship, Drug Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / growth & development* Ecdysone / metabolism* Ecdysterone / chemistry* Female Gene Expression Regulation, Developmental* Juvenile Hormones / antagonists & inhibitors* Juvenile Hormones / metabolism* Larva / metabolism Male Metamorphosis, Biological Pupa / metabolism Signal Transduction Time Factors
IF 2.896
引用数 16
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ DGRC#106364 DGRC#105669 DGRC#106349